Community ecology of hot spring cyanobacterial mats: Predominant populations and their functional potential

Klatt, CG; Wood, JM; Rusch, DB; Bateson, MM; Hamamura, N; Heidelberg, JF; Grossman, AR; Bhaya, D; Cohan, FM; Kühl, M; Bryant, DA; Ward, DM

Community ecology of hot spring cyanobacterial mats: Predominant populations and their functional potential

Klatt, CG Wood, JM Rusch, DB Bateson, MM Hamamura, N Heidelberg, JF Grossman, AR Bhaya, D Cohan, FM Kühl, M

Bryant, DA Ward, DM

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Publication Type:: Journal Article
Citation:: ISME Journal, 2011, 5 (8), pp. 1262 - 1278
Issue Date:: 2011-08-01

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Field	Value	Language
dc.contributor.author	Klatt, CG	en_US
dc.contributor.author	Wood, JM	en_US
dc.contributor.author	Rusch, DB	en_US
dc.contributor.author	Bateson, MM	en_US
dc.contributor.author	Hamamura, N	en_US
dc.contributor.author	Heidelberg, JF	en_US
dc.contributor.author	Grossman, AR	en_US
dc.contributor.author	Bhaya, D	en_US
dc.contributor.author	Cohan, FM	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Bryant, DA	en_US
dc.contributor.author	Ward, DM	en_US
dc.date.issued	2011-08-01	en_US
dc.identifier.citation	ISME Journal, 2011, 5 (8), pp. 1262 - 1278	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14889
dc.description.abstract	Phototrophic microbial mat communities from 60 °C and 65 °C regions in the effluent channels of Mushroom and Octopus Springs (Yellowstone National Park, WY, USA) were investigated by shotgun metagenomic sequencing. Analyses of assembled metagenomic sequences resolved six dominant chlorophototrophic populations and permitted the discovery and characterization of undescribed but predominant community members and their physiological potential. Linkage of phylogenetic marker genes and functional genes showed novel chlorophototrophic bacteria belonging to uncharacterized lineages within the order Chlorobiales and within the Kingdom Chloroflexi. The latter is the first chlorophototrophic member of Kingdom Chloroflexi that lies outside the monophyletic group of chlorophototrophs of the Order Chloroflexales. Direct comparison of unassembled metagenomic sequences to genomes of representative isolates showed extensive genetic diversity, genomic rearrangements and novel physiological potential in native populations as compared with genomic references. Synechococcus spp. metagenomic sequences showed a high degree of synteny with the reference genomes of Synechococcus spp. strains A and B′, but synteny declined with decreasing sequence relatedness to these references. There was evidence of horizontal gene transfer among native populations, but the frequency of these events was inversely proportional to phylogenetic relatedness. © 2011 International Society for Microbial Ecology All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2011.73	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chlorobi	en_US
dc.subject.mesh	Chloroflexi	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Synechococcus	en_US
dc.subject.mesh	Hot Springs	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Gene Transfer, Horizontal	en_US
dc.subject.mesh	United States	en_US
dc.subject.mesh	Metagenome	en_US
dc.title	Community ecology of hot spring cyanobacterial mats: Predominant populations and their functional potential	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	5	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0602 Ecology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Phototrophic microbial mat communities from 60 °C and 65 °C regions in the effluent channels of Mushroom and Octopus Springs (Yellowstone National Park, WY, USA) were investigated by shotgun metagenomic sequencing. Analyses of assembled metagenomic sequences resolved six dominant chlorophototrophic populations and permitted the discovery and characterization of undescribed but predominant community members and their physiological potential. Linkage of phylogenetic marker genes and functional genes showed novel chlorophototrophic bacteria belonging to uncharacterized lineages within the order Chlorobiales and within the Kingdom Chloroflexi. The latter is the first chlorophototrophic member of Kingdom Chloroflexi that lies outside the monophyletic group of chlorophototrophs of the Order Chloroflexales. Direct comparison of unassembled metagenomic sequences to genomes of representative isolates showed extensive genetic diversity, genomic rearrangements and novel physiological potential in native populations as compared with genomic references. Synechococcus spp. metagenomic sequences showed a high degree of synteny with the reference genomes of Synechococcus spp. strains A and B′, but synteny declined with decreasing sequence relatedness to these references. There was evidence of horizontal gene transfer among native populations, but the frequency of these events was inversely proportional to phylogenetic relatedness. © 2011 International Society for Microbial Ecology All rights reserved.

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http://hdl.handle.net/10453/14889