In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS

Jensen, SI; Steunou, AS; Bhaya, D; Kühl, M; Grossman, AR

In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS

Jensen, SI Steunou, AS Bhaya, D Kühl, M

Grossman, AR

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Publication Type:: Journal Article
Citation:: ISME Journal, 2011, 5 (2), pp. 317 - 328
Issue Date:: 2011-02-01

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Field	Value	Language
dc.contributor.author	Jensen, SI	en_US
dc.contributor.author	Steunou, AS	en_US
dc.contributor.author	Bhaya, D	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Grossman, AR	en_US
dc.date.issued	2011-02-01	en_US
dc.identifier.citation	ISME Journal, 2011, 5 (2), pp. 317 - 328	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14909
dc.description.abstract	The relative abundance of transcripts encoding proteins involved in inorganic carbon concentrating mechanisms (CCM), detoxification of reactive oxygen species (ROS) and photosynthesis in the thermophilic cyanobacterium Synechococcus OS-B′ was measured in hot spring microbial mats over two diel cycles, and was coupled with in situ determinations of incoming irradiance and microenvironmental dynamics of O2 and pH. Fluctuations in pH and O2 in the mats were largely driven by the diel cycle of solar irradiance, with a pH variation from ∼7.0 to ∼9.5, and O2 levels ranging from anoxia to supersaturation during night and day, respectively. Levels of various transcripts from mat cyanobacteria revealed several patterns that correlated with incident irradiance, O2 and pH within the mat matrix. Transcript abundances for most genes increased during the morning dark-light transition. Some transcripts remained at a near constant level throughout the light period, whereas others showed an additional increase in abundance as the mat underwent transition from low-to-high light (potentially reflecting changes in O2 concentration and pH), followed by either a decreased abundance in the early afternoon, or a gradual decline during the early afternoon and into the evening. One specific transcipt, psbA1, was the lowest during mid-day under high irradiance and increased when the light levels declined. We discuss these complex in situ transcriptional patterns with respect to environmental and endogenous cues that might impact and regulate transcription over the diel cycle. © 2011 International Society for Microbial Ecology All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2010.131	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Synechococcus	en_US
dc.subject.mesh	Carbon Dioxide	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Reactive Oxygen Species	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Water Microbiology	en_US
dc.subject.mesh	Solar Energy	en_US
dc.subject.mesh	Hot Springs	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.title	In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	5	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0602 Ecology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

The relative abundance of transcripts encoding proteins involved in inorganic carbon concentrating mechanisms (CCM), detoxification of reactive oxygen species (ROS) and photosynthesis in the thermophilic cyanobacterium Synechococcus OS-B′ was measured in hot spring microbial mats over two diel cycles, and was coupled with in situ determinations of incoming irradiance and microenvironmental dynamics of O2 and pH. Fluctuations in pH and O2 in the mats were largely driven by the diel cycle of solar irradiance, with a pH variation from ∼7.0 to ∼9.5, and O2 levels ranging from anoxia to supersaturation during night and day, respectively. Levels of various transcripts from mat cyanobacteria revealed several patterns that correlated with incident irradiance, O2 and pH within the mat matrix. Transcript abundances for most genes increased during the morning dark-light transition. Some transcripts remained at a near constant level throughout the light period, whereas others showed an additional increase in abundance as the mat underwent transition from low-to-high light (potentially reflecting changes in O2 concentration and pH), followed by either a decreased abundance in the early afternoon, or a gradual decline during the early afternoon and into the evening. One specific transcipt, psbA1, was the lowest during mid-day under high irradiance and increased when the light levels declined. We discuss these complex in situ transcriptional patterns with respect to environmental and endogenous cues that might impact and regulate transcription over the diel cycle. © 2011 International Society for Microbial Ecology All rights reserved.

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http://hdl.handle.net/10453/14909