A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata.

Ellis, J; Barratt, J; Kaufer, A; Pearn, L; Armstrong, B; Johnson, M; Park, Y; Downey, L; Cao, M; Neill, L; Lee, R; Ellis, B; Tyler, K; Lun, Z-R; Stark, D

A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata.

Ellis, J

Barratt, J

Kaufer, A Pearn, L Armstrong, B Johnson, M Park, Y Downey, L Cao, M Neill, L Lee, R Ellis, B Tyler, K Lun, Z-R Stark, D

Permalink

Publisher:: Cambridge University Press (CUP)
Publication Type:: Journal Article
Citation:: Parasitology, 2021, pp. 1-12
Issue Date:: 2021-04-12

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (920.27 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831
dc.contributor.author	Barratt, J https://orcid.org/0000-0001-8711-2408
dc.contributor.author	Kaufer, A
dc.contributor.author	Pearn, L
dc.contributor.author	Armstrong, B
dc.contributor.author	Johnson, M
dc.contributor.author	Park, Y
dc.contributor.author	Downey, L
dc.contributor.author	Cao, M
dc.contributor.author	Neill, L
dc.contributor.author	Lee, R
dc.contributor.author	Ellis, B
dc.contributor.author	Tyler, K
dc.contributor.author	Lun, Z-R
dc.contributor.author	Stark, D
dc.date.accessioned	2021-06-04T01:54:41Z
dc.date.available	2021-06-04T01:54:41Z
dc.date.issued	2021-04-12
dc.identifier.citation	Parasitology, 2021, pp. 1-12
dc.identifier.issn	0031-1820
dc.identifier.issn	1469-8161
dc.identifier.uri	http://hdl.handle.net/10453/149408
dc.description.abstract	Previously, it was suggested that haemadipsid leeches represent an important vector of trypanosomes amongst native animals in Australia. Consequently, Chtonobdella bilineata leeches were investigated for the presence of trypanosome species by polymerase chain reaction (PCR), DNA sequencing and in vitro isolation. Phylogenetic analysis ensued to further define the populations present. PCR targeting the 28S rDNA demonstrated that over 95% of C. bilineata contained trypanosomes; diversity profiling by deep amplicon sequencing of 18S rDNA indicated the presence of four different clusters related to the Trypanosoma (Megatrypanum) theileri. Novy–MacNeal–Nicolle slopes with liquid overlay were used to isolate trypanosomes into culture that proved similar in morphology to Trypanosoma cyclops in that they contained a large numbers of acidocalcisomes. Phylogeny of 18S rDNA/GAPDH/ND5 DNA sequences from primary cultures and subclones showed the trypanosomes were monophyletic, with T. cyclops as a sister group. Blood-meal analysis of leeches showed that leeches primarily contained blood from swamp wallaby (Wallabia bicolour), human (Homo sapiens) or horse (Equus sp.). The leech C. bilineata is a host for at least five lineages of Trypanosoma sp. and these are monophyletic with T. cyclops; we propose Trypanosoma cyclops australiensis as a subspecies of T. cyclops based on genetic similarity and biogeography considerations.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Cambridge University Press (CUP)
dc.relation.ispartof	Parasitology
dc.relation.isbasedon	10.1017/s0031182021000639
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0707 Veterinary Sciences
dc.subject.classification	Mycology & Parasitology
dc.title	A new subspecies of <i>Trypanosoma cyclops</i> found in the Australian terrestrial leech <i>Chtonobdella bilineata</i>.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-06-04T01:54:39Z
pubs.publication-status	Published

Abstract:

Previously, it was suggested that haemadipsid leeches represent an important vector of trypanosomes amongst native animals in Australia. Consequently, Chtonobdella bilineata leeches were investigated for the presence of trypanosome species by polymerase chain reaction (PCR), DNA sequencing and in vitro isolation. Phylogenetic analysis ensued to further define the populations present. PCR targeting the 28S rDNA demonstrated that over 95% of C. bilineata contained trypanosomes; diversity profiling by deep amplicon sequencing of 18S rDNA indicated the presence of four different clusters related to the Trypanosoma (Megatrypanum) theileri. Novy–MacNeal–Nicolle slopes with liquid overlay were used to isolate trypanosomes into culture that proved similar in morphology to Trypanosoma cyclops in that they contained a large numbers of acidocalcisomes. Phylogeny of 18S rDNA/GAPDH/ND5 DNA sequences from primary cultures and subclones showed the trypanosomes were monophyletic, with T. cyclops as a sister group. Blood-meal analysis of leeches showed that leeches primarily contained blood from swamp wallaby (Wallabia bicolour), human (Homo sapiens) or horse (Equus sp.). The leech C. bilineata is a host for at least five lineages of Trypanosoma sp. and these are monophyletic with T. cyclops; we propose Trypanosoma cyclops australiensis as a subspecies of T. cyclops based on genetic similarity and biogeography considerations.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/149408

A new subspecies of <i>Trypanosoma cyclops</i> found in the Australian terrestrial leech <i>Chtonobdella bilineata</i>.