The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Jannot, G; Bajan, S; Giguère, NJ; Bouasker, S; Banville, IH; Piquet, S; Hutvagner, G; Simard, MJ

The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Jannot, G Bajan, S

Giguère, NJ Bouasker, S Banville, IH Piquet, S Hutvagner, G

Simard, MJ

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Publication Type:: Journal Article
Citation:: EMBO Reports, 2011, 12 (6), pp. 581 - 586
Issue Date:: 2011-06-01

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Field	Value	Language
dc.contributor.author	Jannot, G	en_US
dc.contributor.author	Bajan, S https://orcid.org/0000-0001-6934-5240	en_US
dc.contributor.author	Giguère, NJ	en_US
dc.contributor.author	Bouasker, S	en_US
dc.contributor.author	Banville, IH	en_US
dc.contributor.author	Piquet, S	en_US
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	Simard, MJ	en_US
dc.date.available	2011-03-23	en_US
dc.date.issued	2011-06-01	en_US
dc.identifier.citation	EMBO Reports, 2011, 12 (6), pp. 581 - 586	en_US
dc.identifier.issn	1469-221X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14967
dc.description.abstract	Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. © 2011 European Molecular Biology Organization.	en_US
dc.relation.ispartof	EMBO Reports	en_US
dc.relation.isbasedon	10.1038/embor.2011.66	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Polyribosomes	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Caenorhabditis elegans	en_US
dc.subject.mesh	GTP-Binding Proteins	en_US
dc.subject.mesh	RNA-Induced Silencing Complex	en_US
dc.subject.mesh	Caenorhabditis elegans Proteins	en_US
dc.subject.mesh	Receptors, Cell Surface	en_US
dc.subject.mesh	Neoplasm Proteins	en_US
dc.subject.mesh	Receptors, Cytoplasmic and Nuclear	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Gene Silencing	en_US
dc.subject.mesh	Protein Binding	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.subject.mesh	Receptors for Activated C Kinase	en_US
dc.title	The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	12	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
dc.location.activity	ISI:000291148200020	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	12	en_US

Abstract:

Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. © 2011 European Molecular Biology Organization.

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http://hdl.handle.net/10453/14967