The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans
- Publication Type:
- Journal Article
- Citation:
- EMBO Reports, 2011, 12 (6), pp. 581 - 586
- Issue Date:
- 2011-06-01
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Jannot, G | en_US |
dc.contributor.author |
Bajan, S https://orcid.org/0000-0001-6934-5240 |
en_US |
dc.contributor.author | Giguère, NJ | en_US |
dc.contributor.author | Bouasker, S | en_US |
dc.contributor.author | Banville, IH | en_US |
dc.contributor.author | Piquet, S | en_US |
dc.contributor.author |
Hutvagner, G https://orcid.org/0000-0002-7231-9446 |
en_US |
dc.contributor.author | Simard, MJ | en_US |
dc.date.available | 2011-03-23 | en_US |
dc.date.issued | 2011-06-01 | en_US |
dc.identifier.citation | EMBO Reports, 2011, 12 (6), pp. 581 - 586 | en_US |
dc.identifier.issn | 1469-221X | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/14967 | |
dc.description.abstract | Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. © 2011 European Molecular Biology Organization. | en_US |
dc.relation.ispartof | EMBO Reports | en_US |
dc.relation.isbasedon | 10.1038/embor.2011.66 | en_US |
dc.subject.classification | Developmental Biology | en_US |
dc.subject.mesh | Hela Cells | en_US |
dc.subject.mesh | Polyribosomes | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Caenorhabditis elegans | en_US |
dc.subject.mesh | GTP-Binding Proteins | en_US |
dc.subject.mesh | RNA-Induced Silencing Complex | en_US |
dc.subject.mesh | Caenorhabditis elegans Proteins | en_US |
dc.subject.mesh | Receptors, Cell Surface | en_US |
dc.subject.mesh | Neoplasm Proteins | en_US |
dc.subject.mesh | Receptors, Cytoplasmic and Nuclear | en_US |
dc.subject.mesh | MicroRNAs | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Gene Silencing | en_US |
dc.subject.mesh | Protein Binding | en_US |
dc.subject.mesh | HeLa Cells | en_US |
dc.subject.mesh | Receptors for Activated C Kinase | en_US |
dc.title | The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 6 | en_US |
utslib.citation.volume | 12 | en_US |
utslib.for | 0903 Biomedical Engineering | en_US |
utslib.for | 0601 Biochemistry and Cell Biology | en_US |
dc.location.activity | ISI:000291148200020 | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
utslib.copyright.status | open_access | |
pubs.issue | 6 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 12 | en_US |
Abstract:
Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. © 2011 European Molecular Biology Organization.
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