Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland)

Roeselers, G; Norris, TB; Castenholz, RW; Rysgaard, S; Glud, RN; Kühl, M; Muyzer, G

Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland)

Roeselers, G Norris, TB Castenholz, RW Rysgaard, S Glud, RN Kühl, M

Muyzer, G

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2007, 9 (1), pp. 26 - 38
Issue Date:: 2007-01-01

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Field	Value	Language
dc.contributor.author	Roeselers, G	en_US
dc.contributor.author	Norris, TB	en_US
dc.contributor.author	Castenholz, RW	en_US
dc.contributor.author	Rysgaard, S	en_US
dc.contributor.author	Glud, RN	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Muyzer, G	en_US
dc.date.issued	2007-01-01	en_US
dc.identifier.citation	Environmental Microbiology, 2007, 9 (1), pp. 26 - 38	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14981
dc.description.abstract	We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime. © 2006 The Authors.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/j.1462-2920.2006.01103.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chlorobi	en_US
dc.subject.mesh	Chloroflexi	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Chromatiaceae	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Hot Springs	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Greenland	en_US
dc.subject.mesh	Arctic Regions	en_US
dc.subject.mesh	Phototrophic Processes	en_US
dc.title	Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland)	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	9	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime. © 2006 The Authors.

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http://hdl.handle.net/10453/14981