Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System.

Aksoy, YA; Yang, B; Chen, W; Hung, T; Kuchel, RP; Zammit, NW; Grey, ST; Goldys, EM; Deng, W

Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System.

Aksoy, YA Yang, B Chen, W Hung, T Kuchel, RP Zammit, NW Grey, ST Goldys, EM Deng, W

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS applied materials & interfaces, 2020, 12, (47), pp. 52433-52444
Issue Date:: 2020-11-11

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Field	Value	Language
dc.contributor.author	Aksoy, YA
dc.contributor.author	Yang, B
dc.contributor.author	Chen, W
dc.contributor.author	Hung, T
dc.contributor.author	Kuchel, RP
dc.contributor.author	Zammit, NW
dc.contributor.author	Grey, ST
dc.contributor.author	Goldys, EM
dc.contributor.author	Deng, W https://orcid.org/0000-0002-9413-0978
dc.date.accessioned	2021-07-04T23:54:20Z
dc.date.available	2021-07-04T23:54:20Z
dc.date.issued	2020-11-11
dc.identifier.citation	ACS applied materials & interfaces, 2020, 12, (47), pp. 52433-52444
dc.identifier.issn	1944-8244
dc.identifier.issn	1944-8252
dc.identifier.uri	http://hdl.handle.net/10453/149817
dc.description.abstract	The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an <i>in vivo</i> eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	ACS applied materials & interfaces
dc.relation.isbasedon	10.1021/acsami.0c16380
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Embryo, Nonmammalian
dc.subject.mesh	Animals
dc.subject.mesh	Zebrafish
dc.subject.mesh	Humans
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Tumor Necrosis Factor-alpha
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	Liposomes
dc.subject.mesh	Gene Expression
dc.subject.mesh	Genes, Reporter
dc.subject.mesh	Light
dc.subject.mesh	HEK293 Cells
dc.subject.mesh	CRISPR-Cas Systems
dc.subject.mesh	Tumor Necrosis Factor alpha-Induced Protein 3
dc.subject.mesh	Gene Editing
dc.subject.mesh	Animals
dc.subject.mesh	CRISPR-Cas Systems
dc.subject.mesh	Embryo, Nonmammalian
dc.subject.mesh	Gene Editing
dc.subject.mesh	Gene Expression
dc.subject.mesh	Genes, Reporter
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	HEK293 Cells
dc.subject.mesh	Humans
dc.subject.mesh	Light
dc.subject.mesh	Liposomes
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Tumor Necrosis Factor alpha-Induced Protein 3
dc.subject.mesh	Tumor Necrosis Factor-alpha
dc.subject.mesh	Zebrafish
dc.title	Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	United States
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	0903 Biomedical Engineering
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2021-07-04T23:54:17Z
pubs.issue	47
pubs.publication-status	Published
pubs.volume	12
utslib.citation.issue	47

Abstract:

The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.

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http://hdl.handle.net/10453/149817