Seaweed Laminaria japonica peptides possess strong anti-liver cancer effects

Chen, H; Wu, Y; Chen, Y; Li, Y; McGowan, E; Lin, Y

Seaweed Laminaria japonica peptides possess strong anti-liver cancer effects

Chen, H Wu, Y Chen, Y Li, Y McGowan, E Lin, Y

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Publisher:: Elsevier
Publication Type:: Conference Proceeding
Citation:: Annals of Oncology, 2021, 32, (Supplement 3), pp. S189-S189
Issue Date:: 2021-06-30

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Field	Value	Language
dc.contributor.author	Chen, H
dc.contributor.author	Wu, Y
dc.contributor.author	Chen, Y
dc.contributor.author	Li, Y
dc.contributor.author	McGowan, E
dc.contributor.author	Lin, Y https://orcid.org/0000-0002-4637-9701
dc.date	2021-06-30
dc.date.accessioned	2021-07-06T13:30:44Z
dc.date.available	2021-04-16
dc.date.available	2021-07-06T13:30:44Z
dc.date.issued	2021-06-30
dc.identifier.citation	Annals of Oncology, 2021, 32, (Supplement 3), pp. S189-S189
dc.identifier.issn	0923-7534
dc.identifier.uri	http://hdl.handle.net/10453/149840
dc.description.abstract	Background: Seaweed Laminaria japonica is widely consumed worldwide. This seaweed has also been used in traditional Chinese medicine for hundreds of years. Recent studies have shown that Laminaria japonica has multiple beneficial effects for human health, however, little is known about its anti-tumour effects. In the current study, we extracted Laminaria japonica peptides (LJPs) using an activity tracking method and explored the anti-tumour (liver cancer) activity of the peptides both in vivo and in vitro. Methods: The LJP’s were isolated with fresh Laminaria japonica and purified by Reversed Phase-High Performance Liquid Chromatography and HPLC-ESI-MS. Liver cancer cell lines HuH7, HepG2 cells and the human liver cell line HL-7702 cells were used throughout the experiment. The HepG2 cells were treated with LJP-1 (0, 0.36 mM and 1.80 mM) for 24 h, and cell apoptosis and cell cycle were evaluated. MTT assay was used to examine the cytotoxicity activity of the LJPs. Pro-apoptotic factors (including. Bax, Bid) and anti-apoptotic factors (including, Bcl-2, Bcl-xL) were investigated in cells treated with LJP. Caspase activation was examined by detecting the protein expressions of Caspase 3, Caspase 9, Cleaved caspase 3, Cleaved caspase 9 and Cleaved PARP using western blot. The phosphatidylinositol-3-kinase (PI3K)/AKT pathway and the phosphorylation states of the MAPKs (p38 and JNK) were also examined. The in vivo anti-liver cancer effect of LJP was investigated using a mouse model. Results: Results showed that LJP-1 [EGFHL] performed the most anti-proliferative activity in H22 cells in vitro. LJP-1 blocked the H22 cells in G0/G1 phase accompanied by inhibition of cyclins expression. Further experiments revealed that LJP-1 induced H22 cell apoptosis via Caspase activation and ASK1/MAPKs pathway regulation. At the same time, LJP-1 significantly inhibited the tumour growth and induced tumour cell apoptosis and necrosis in vivo. Conclusions: Our results support that the peptides (LJP’s) from Laminaria japonica possess strong inhibitory effects on the growth of liver cancer both in vivo and in vitro. LJP-1 induces HCC cell apoptosis through the Caspase-dependent pathway and via G0/G1 phase arrest by regulating cell cycle checkpoint proteins. Additionally, LJP-1 induces Caspase dependent apoptosis, in part by inhibiting the PI3K/AKT and MAPK signaling pathways. Potentially LJP-1 could be a novel candidate for human liver cancer therapeutics.
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Annals of Oncology
dc.relation.ispartof	The 22nd World Congress on Gastrointestinal Cancer
dc.relation.isbasedon	10.1016/j.annonc.2021.05.322
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1112 Oncology and Carcinogenesis
dc.subject.classification	Oncology & Carcinogenesis
dc.title	Seaweed Laminaria japonica peptides possess strong anti-liver cancer effects
dc.type	Conference Proceeding
utslib.citation.volume	32
utslib.location.activity	Virtual
utslib.for	1112 Oncology and Carcinogenesis
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	true
dc.date.updated	2021-07-06T13:30:42Z
pubs.finish-date	2021-07-03
pubs.issue	Supplement 3
pubs.publication-status	Published
pubs.start-date	2021-06-30
pubs.volume	32
utslib.citation.issue	Supplement 3

Abstract:

Background: Seaweed Laminaria japonica is widely consumed worldwide. This seaweed has also been used in traditional Chinese medicine for hundreds of years. Recent studies have shown that Laminaria japonica has multiple beneficial effects for human health, however, little is known about its anti-tumour effects. In the current study, we extracted Laminaria japonica peptides (LJPs) using an activity tracking method and explored the anti-tumour (liver cancer) activity of the peptides both in vivo and in vitro. Methods: The LJP’s were isolated with fresh Laminaria japonica and purified by Reversed Phase-High Performance Liquid Chromatography and HPLC-ESI-MS. Liver cancer cell lines HuH7, HepG2 cells and the human liver cell line HL-7702 cells were used throughout the experiment. The HepG2 cells were treated with LJP-1 (0, 0.36 mM and 1.80 mM) for 24 h, and cell apoptosis and cell cycle were evaluated. MTT assay was used to examine the cytotoxicity activity of the LJPs. Pro-apoptotic factors (including. Bax, Bid) and anti-apoptotic factors (including, Bcl-2, Bcl-xL) were investigated in cells treated with LJP. Caspase activation was examined by detecting the protein expressions of Caspase 3, Caspase 9, Cleaved caspase 3, Cleaved caspase 9 and Cleaved PARP using western blot. The phosphatidylinositol-3-kinase (PI3K)/AKT pathway and the phosphorylation states of the MAPKs (p38 and JNK) were also examined. The in vivo anti-liver cancer effect of LJP was investigated using a mouse model. Results: Results showed that LJP-1 [EGFHL] performed the most anti-proliferative activity in H22 cells in vitro. LJP-1 blocked the H22 cells in G0/G1 phase accompanied by inhibition of cyclins expression. Further experiments revealed that LJP-1 induced H22 cell apoptosis via Caspase activation and ASK1/MAPKs pathway regulation. At the same time, LJP-1 significantly inhibited the tumour growth and induced tumour cell apoptosis and necrosis in vivo. Conclusions: Our results support that the peptides (LJP’s) from Laminaria japonica possess strong inhibitory effects on the growth of liver cancer both in vivo and in vitro. LJP-1 induces HCC cell apoptosis through the Caspase-dependent pathway and via G0/G1 phase arrest by regulating cell cycle checkpoint proteins. Additionally, LJP-1 induces Caspase dependent apoptosis, in part by inhibiting the PI3K/AKT and MAPK signaling pathways. Potentially LJP-1 could be a novel candidate for human liver cancer therapeutics.

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http://hdl.handle.net/10453/149840