Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: A comparative study

Chowdhury, PR; Ingold, A; Vanegas, N; Martínez, E; Merlino, J; Merkier, AK; Castro, M; Rocha, GG; Borthagaray, G; Centrón, D; Toledo, HB; Márquez, CM; Stokes, HW

Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: A comparative study

Ingold, A Vanegas, N Martínez, E Merlino, J Merkier, AK Castro, M Rocha, GG Borthagaray, G Centrón, D Toledo, HB Márquez, CM Stokes, HW

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Publication Type:: Journal Article
Citation:: Antimicrobial Agents and Chemotherapy, 2011, 55 (7), pp. 3140 - 3149
Issue Date:: 2011-07-01

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Field	Value	Language
dc.contributor.author	Chowdhury, PR https://orcid.org/0000-0003-4352-603X	en_US
dc.contributor.author	Ingold, A	en_US
dc.contributor.author	Vanegas, N	en_US
dc.contributor.author	Martínez, E	en_US
dc.contributor.author	Merlino, J	en_US
dc.contributor.author	Merkier, AK	en_US
dc.contributor.author	Castro, M	en_US
dc.contributor.author	Rocha, GG	en_US
dc.contributor.author	Borthagaray, G	en_US
dc.contributor.author	Centrón, D	en_US
dc.contributor.author	Toledo, HB	en_US
dc.contributor.author	Márquez, CM	en_US
dc.contributor.author	Stokes, HW	en_US
dc.date.issued	2011-07-01	en_US
dc.identifier.citation	Antimicrobial Agents and Chemotherapy, 2011, 55 (7), pp. 3140 - 3149	en_US
dc.identifier.issn	0066-4804	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14987
dc.description.abstract	A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging to the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on the genetic contexts of class 1 integrons, mobilizable genetic elements best known for their role in the rapid evolution of antibiotic resistance among Gram-negative pathogens. It was found that the class 1 integrons in this cohort were located in a number of different genetic contexts with clear regional differences. In Sydney, IS26-associated Tn21-like transposons on IncL/M plasmids contribute greatly to the dispersal of integron-associated multiple-drug-resistant (MDR) loci. In contrast, in the South American countries, Tn1696-like transposons on an IncA/C plasmid(s) appeared to be disseminating a characteristic MDR region. A range of mobile genetic elements is clearly being recruited by clinically important mobile class 1 integrons, and these elements appear to be becoming more common with time. This in turn is driving the evolution of complex and laterally mobile MDR units and may further complicate antibiotic therapy. Copyright © 2011, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Antimicrobial Agents and Chemotherapy	en_US
dc.relation.isbasedon	10.1128/AAC.01529-10	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Klebsiella pneumoniae	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Electrophoresis, Gel, Pulsed-Field	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Integrons	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: A comparative study	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	55	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	55	en_US

Abstract:

A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging to the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on the genetic contexts of class 1 integrons, mobilizable genetic elements best known for their role in the rapid evolution of antibiotic resistance among Gram-negative pathogens. It was found that the class 1 integrons in this cohort were located in a number of different genetic contexts with clear regional differences. In Sydney, IS26-associated Tn21-like transposons on IncL/M plasmids contribute greatly to the dispersal of integron-associated multiple-drug-resistant (MDR) loci. In contrast, in the South American countries, Tn1696-like transposons on an IncA/C plasmid(s) appeared to be disseminating a characteristic MDR region. A range of mobile genetic elements is clearly being recruited by clinically important mobile class 1 integrons, and these elements appear to be becoming more common with time. This in turn is driving the evolution of complex and laterally mobile MDR units and may further complicate antibiotic therapy. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/14987