Assessing coral sperm motility.

Zuchowicz, N; Daly, J; Bouwmeester, J; Lager, C; Henley, EM; Nuñez Lendo, CI; Hagedorn, M

Assessing coral sperm motility.

Zuchowicz, N Daly, J Bouwmeester, J Lager, C Henley, EM Nuñez Lendo, CI Hagedorn, M

Permalink

Publisher:: NATURE RESEARCH
Publication Type:: Journal Article
Citation:: Scientific reports, 2021, 11, (1), pp. 61
Issue Date:: 2021-01-08

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (1.15 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Zuchowicz, N
dc.contributor.author	Daly, J
dc.contributor.author	Bouwmeester, J
dc.contributor.author	Lager, C
dc.contributor.author	Henley, EM
dc.contributor.author	Nuñez Lendo, CI
dc.contributor.author	Hagedorn, M
dc.date.accessioned	2021-10-16T22:18:27Z
dc.date.available	2020-12-07
dc.date.available	2021-10-16T22:18:27Z
dc.date.issued	2021-01-08
dc.identifier.citation	Scientific reports, 2021, 11, (1), pp. 61
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/151117
dc.description.abstract	The declining reproductive viability of corals threatens their ability to adapt to changing ocean conditions. It is vital that we monitor this viability quantitatively and comparatively. Computer-assisted sperm analysis (CASA) systems offer in-depth analysis used regularly for domestic and wildlife species, but not yet for coral. This study proposes quality control procedures and CASA settings that are effective for coral sperm analysis. To resolve disparities between CASA measurements and evaluations by eye, two negative effects on motility had to be resolved, slide adhesion (procedural) and sperm dilution (biological). We showed that the addition of bovine serum albumin, or caffeine, or both to fresh sperm reduced adhesion in the CASA cassettes, improved motility and motile sperm concentration (P < 0.0001), yet these additions did not affect measurements of total sperm concentration. Diluting coral sperm reduced sperm motility (P = 0.039), especially from heat-stressed corals. We found CASA concentration counts comparable to haemocytometer and flow cytometer measures (P = 0.54). We also found that motile sperm per egg is a useful predictor of fertilisation success, using cryopreserved sperm. Standard measurements of coral reproductive characteristics inform our understanding of the impacts of climate change on reef populations; this study provides a benchmark to begin this comparative work.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE RESEARCH
dc.relation.ispartof	Scientific reports
dc.relation.isbasedon	10.1038/s41598-020-79732-x
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Spermatozoa
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Sperm Motility
dc.subject.mesh	Male
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Male
dc.subject.mesh	Sperm Motility
dc.subject.mesh	Spermatozoa
dc.title	Assessing coral sperm motility.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-10-16T22:18:23Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

The declining reproductive viability of corals threatens their ability to adapt to changing ocean conditions. It is vital that we monitor this viability quantitatively and comparatively. Computer-assisted sperm analysis (CASA) systems offer in-depth analysis used regularly for domestic and wildlife species, but not yet for coral. This study proposes quality control procedures and CASA settings that are effective for coral sperm analysis. To resolve disparities between CASA measurements and evaluations by eye, two negative effects on motility had to be resolved, slide adhesion (procedural) and sperm dilution (biological). We showed that the addition of bovine serum albumin, or caffeine, or both to fresh sperm reduced adhesion in the CASA cassettes, improved motility and motile sperm concentration (P < 0.0001), yet these additions did not affect measurements of total sperm concentration. Diluting coral sperm reduced sperm motility (P = 0.039), especially from heat-stressed corals. We found CASA concentration counts comparable to haemocytometer and flow cytometer measures (P = 0.54). We also found that motile sperm per egg is a useful predictor of fertilisation success, using cryopreserved sperm. Standard measurements of coral reproductive characteristics inform our understanding of the impacts of climate change on reef populations; this study provides a benchmark to begin this comparative work.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/151117