Differential inflammatory and toxic effects in-vitro of wood smoke and traffic-related particulate matter from Sydney, Australia.

Wang, B; Chen, H; Xenaki, D; Liao, J; Cowie, C; Oliver, BG

Differential inflammatory and toxic effects in-vitro of wood smoke and traffic-related particulate matter from Sydney, Australia.

Wang, B

Chen, H

Xenaki, D Liao, J Cowie, C Oliver, BG

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Chemosphere, 2021, 272, pp. 129616
Issue Date:: 2021-06

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Field	Value	Language
dc.contributor.author	Wang, B https://orcid.org/0000-0002-8701-3896
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752
dc.contributor.author	Xenaki, D
dc.contributor.author	Liao, J
dc.contributor.author	Cowie, C
dc.contributor.author	Oliver, BG
dc.date.accessioned	2021-10-28T07:08:47Z
dc.date.available	2021-01-08
dc.date.available	2021-10-28T07:08:47Z
dc.date.issued	2021-06
dc.identifier.citation	Chemosphere, 2021, 272, pp. 129616
dc.identifier.issn	0045-6535
dc.identifier.issn	1879-1298
dc.identifier.uri	http://hdl.handle.net/10453/151254
dc.description.abstract	<h4>Background</h4>It is well known that PM<sub>2.5</sub> generated by traffic or burning wood is pro-inflammatory and induces various adverse health outcomes in humans. In Sydney, New South Wales, Australia, the main anthropogenic contributors to particulate matter (PM) air pollution are wood combustion heaters, on-road vehicles, and coal-fired power stations. However, the relative toxicity of these local sources has not to date been investigated.<h4>Method</h4>PM<sub>2.5</sub> was collected on filters from the same sampling site in Liverpool, one suburb of Sydney. According to the positive matrix factorisation and collection season, filters were representative of either day with high traffic-related air pollution (TRAP), wood smoke, or both TRAP and woodsmoke (mixed air pollution). The elemental composition of the PM was assessed by accelerator-based ion beam analysis techniques (i.e. PIXE & PIGE) and size by Dynamic Light Scattering. Toxicity and inflammation were assessed in-vitro in human bronchial epithelial cells by measuring interleukin-6 (IL-6), interleukin-8 (IL-8) release, and MTT.<h4>Results</h4>Mixed air pollution (TRAP/wood smoke) PM had more nanometer (nm) sized PM than the other two groups. Using an in-vitro model of the lungs, the mixed air pollution PM was the most toxic, whereas the PM from woodsmoke induced greater IL-6 release than TRAP PM. There was no difference in the induction of IL-8 between the three sources of PM.<h4>Conclusion</h4>Marked differences occur in the cellular response to PM from different sources, with differences in both toxicity and inflammation.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1158186
dc.relation.ispartof	Chemosphere
dc.relation.isbasedon	10.1016/j.chemosphere.2021.129616
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Sciences
dc.subject.classification	Meteorology & Atmospheric Sciences
dc.subject.mesh	Humans
dc.subject.mesh	Air Pollutants
dc.subject.mesh	Smoke
dc.subject.mesh	Wood
dc.subject.mesh	Australia
dc.subject.mesh	New South Wales
dc.subject.mesh	Particulate Matter
dc.subject.mesh	Air Pollutants
dc.subject.mesh	Australia
dc.subject.mesh	Humans
dc.subject.mesh	New South Wales
dc.subject.mesh	Particulate Matter
dc.subject.mesh	Smoke
dc.subject.mesh	Wood
dc.title	Differential inflammatory and toxic effects in-vitro of wood smoke and traffic-related particulate matter from Sydney, Australia.
dc.type	Journal Article
utslib.citation.volume	272
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2021-10-28T07:08:41Z
pubs.publication-status	Published
pubs.volume	272

Abstract:

Background

It is well known that PM_2.5 generated by traffic or burning wood is pro-inflammatory and induces various adverse health outcomes in humans. In Sydney, New South Wales, Australia, the main anthropogenic contributors to particulate matter (PM) air pollution are wood combustion heaters, on-road vehicles, and coal-fired power stations. However, the relative toxicity of these local sources has not to date been investigated.

Method

PM_2.5 was collected on filters from the same sampling site in Liverpool, one suburb of Sydney. According to the positive matrix factorisation and collection season, filters were representative of either day with high traffic-related air pollution (TRAP), wood smoke, or both TRAP and woodsmoke (mixed air pollution). The elemental composition of the PM was assessed by accelerator-based ion beam analysis techniques (i.e. PIXE & PIGE) and size by Dynamic Light Scattering. Toxicity and inflammation were assessed in-vitro in human bronchial epithelial cells by measuring interleukin-6 (IL-6), interleukin-8 (IL-8) release, and MTT.

Results

Mixed air pollution (TRAP/wood smoke) PM had more nanometer (nm) sized PM than the other two groups. Using an in-vitro model of the lungs, the mixed air pollution PM was the most toxic, whereas the PM from woodsmoke induced greater IL-6 release than TRAP PM. There was no difference in the induction of IL-8 between the three sources of PM.

Conclusion

Marked differences occur in the cellular response to PM from different sources, with differences in both toxicity and inflammation.

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http://hdl.handle.net/10453/151254