Proposed Framework for Comparison of Continuous Probabilistic Genotyping Systems amongst Different Laboratories

McNevin, D; Wright, K; Barash, M; Gomes, S; Jamieson, A; Chaseling, J

Proposed Framework for Comparison of Continuous Probabilistic Genotyping Systems amongst Different Laboratories

McNevin, D

Wright, K Barash, M

Gomes, S Jamieson, A Chaseling, J

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Journal of Forensic Sciences, 2021, 1, (1), pp. 33-45
Issue Date:: 2021-06-10

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Field	Value	Language
dc.contributor.author	McNevin, D https://orcid.org/0000-0003-1665-3367
dc.contributor.author	Wright, K
dc.contributor.author	Barash, M https://orcid.org/0000-0002-5445-6316
dc.contributor.author	Gomes, S
dc.contributor.author	Jamieson, A
dc.contributor.author	Chaseling, J
dc.date.accessioned	2021-12-01T02:11:27Z
dc.date.available	2021-12-01T02:11:27Z
dc.date.issued	2021-06-10
dc.identifier.citation	Journal of Forensic Sciences, 2021, 1, (1), pp. 33-45
dc.identifier.issn	0022-1198
dc.identifier.issn	2673-6756
dc.identifier.uri	http://hdl.handle.net/10453/151957
dc.description.abstract	<jats:p>Continuous probabilistic genotyping (PG) systems are becoming the default method for calculating likelihood ratios (LRs) for competing propositions about DNA mixtures. Calculation of the LR relies on numerical methods and simultaneous probabilistic simulations of multiple variables rather than on analytical solutions alone. Some also require modelling of individual laboratory processes that give rise to electropherogram artefacts and peak height variance. For these reasons, it has been argued that any LR produced by continuous PG is unique and cannot be compared with another. We challenge this assumption and demonstrate that there are a set of conditions defining specific DNA mixtures which can produce an aspirational LR and thereby provide a measure of reproducibility for DNA profiling systems incorporating PG. Such DNA mixtures could serve as the basis for inter-laboratory comparisons, even when different STR amplification kits are employed. We propose a procedure for an inter-laboratory comparison consistent with these conditions.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation.ispartof	Journal of Forensic Sciences
dc.relation.isbasedon	10.3390/forensicsci1010006
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Proposed Framework for Comparison of Continuous Probabilistic Genotyping Systems amongst Different Laboratories
dc.type	Journal Article
utslib.citation.volume	1
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-12-01T02:11:25Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	1
utslib.citation.issue	1

Abstract:

Continuous probabilistic genotyping (PG) systems are becoming the default method for calculating likelihood ratios (LRs) for competing propositions about DNA mixtures. Calculation of the LR relies on numerical methods and simultaneous probabilistic simulations of multiple variables rather than on analytical solutions alone. Some also require modelling of individual laboratory processes that give rise to electropherogram artefacts and peak height variance. For these reasons, it has been argued that any LR produced by continuous PG is unique and cannot be compared with another. We challenge this assumption and demonstrate that there are a set of conditions defining specific DNA mixtures which can produce an aspirational LR and thereby provide a measure of reproducibility for DNA profiling systems incorporating PG. Such DNA mixtures could serve as the basis for inter-laboratory comparisons, even when different STR amplification kits are employed. We propose a procedure for an inter-laboratory comparison consistent with these conditions.

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http://hdl.handle.net/10453/151957