Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.

Hoque, MM; Noorian, P; Espinoza-Vergara, G; Manuneedhi Cholan, P; Kim, M; Rahman, MH; Labbate, M; Rice, SA; Pernice, M; Oehlers, SH; McDougald, D

Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.

Hoque, MM Noorian, P

Espinoza-Vergara, G Manuneedhi Cholan, P Kim, M Rahman, MH Labbate, M

Rice, SA Pernice, M

Oehlers, SH McDougald, D

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: The ISME journal, 2021, pp. 1-12
Issue Date:: 2021-10-15

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Field	Value	Language
dc.contributor.author	Hoque, MM
dc.contributor.author	Noorian, P https://orcid.org/0000-0002-4215-1080
dc.contributor.author	Espinoza-Vergara, G
dc.contributor.author	Manuneedhi Cholan, P
dc.contributor.author	Kim, M
dc.contributor.author	Rahman, MH
dc.contributor.author	Labbate, M https://orcid.org/0000-0003-1428-3382
dc.contributor.author	Rice, SA
dc.contributor.author	Pernice, M https://orcid.org/0000-0002-3431-2104
dc.contributor.author	Oehlers, SH
dc.contributor.author	McDougald, D
dc.date.accessioned	2021-12-01T23:15:27Z
dc.date.available	2021-09-29
dc.date.available	2021-12-01T23:15:27Z
dc.date.issued	2021-10-15
dc.identifier.citation	The ISME journal, 2021, pp. 1-12
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/152012
dc.description.abstract	Predation by heterotrophic protists drives the emergence of adaptive traits in bacteria, and often these traits lead to altered interactions with hosts and persistence in the environment. Here we studied adaptation of the cholera pathogen, Vibrio cholerae during long-term co-incubation with the protist host, Acanthamoeba castellanii. We determined phenotypic and genotypic changes associated with long-term intra-amoebal host adaptation and how this impacts pathogen survival and fitness. We showed that adaptation to the amoeba host leads to temporal changes in multiple phenotypic traits in V. cholerae that facilitate increased survival and competitive fitness in amoeba. Genome sequencing and mutational analysis revealed that these altered lifestyles were linked to non-synonymous mutations in conserved regions of the flagellar transcriptional regulator, flrA. Additionally, the mutations resulted in enhanced colonisation in zebrafish, establishing a link between adaptation of V. cholerae to amoeba predation and enhanced environmental persistence. Our results show that pressure imposed by amoeba on V. cholerae selects for flrA mutations that serves as a key driver for adaptation. Importantly, this study provides evidence that adaptive traits that evolve in pathogens in response to environmental predatory pressure impact the colonisation of eukaryotic organisms by these pathogens.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation	http://purl.org/au-research/grants/arc/DP170100453
dc.relation.ispartof	The ISME journal
dc.relation.isbasedon	10.1038/s41396-021-01134-2
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.title	Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-12-01T23:15:26Z
pubs.publication-status	Published

Abstract:

Predation by heterotrophic protists drives the emergence of adaptive traits in bacteria, and often these traits lead to altered interactions with hosts and persistence in the environment. Here we studied adaptation of the cholera pathogen, Vibrio cholerae during long-term co-incubation with the protist host, Acanthamoeba castellanii. We determined phenotypic and genotypic changes associated with long-term intra-amoebal host adaptation and how this impacts pathogen survival and fitness. We showed that adaptation to the amoeba host leads to temporal changes in multiple phenotypic traits in V. cholerae that facilitate increased survival and competitive fitness in amoeba. Genome sequencing and mutational analysis revealed that these altered lifestyles were linked to non-synonymous mutations in conserved regions of the flagellar transcriptional regulator, flrA. Additionally, the mutations resulted in enhanced colonisation in zebrafish, establishing a link between adaptation of V. cholerae to amoeba predation and enhanced environmental persistence. Our results show that pressure imposed by amoeba on V. cholerae selects for flrA mutations that serves as a key driver for adaptation. Importantly, this study provides evidence that adaptive traits that evolve in pathogens in response to environmental predatory pressure impact the colonisation of eukaryotic organisms by these pathogens.

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http://hdl.handle.net/10453/152012