A label-free Exonuclease I-assisted fluorescence aptasensor for highly selective and sensitive detection of silver ions.
- Publisher:
- Elsevier BV
- Publication Type:
- Journal Article
- Citation:
- Spectrochim Acta A Mol Biomol Spectrosc, 2021, 260, pp. 119927
- Issue Date:
- 2021-11-05
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
1-s2.0-S1386142521005047-main.pdf | Published Version | 1.08 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Wei, Z | |
dc.contributor.author | Lan, Y | |
dc.contributor.author | Zhang, C | |
dc.contributor.author | Jia, J | |
dc.contributor.author | Niu, W | |
dc.contributor.author | Wei, Y | |
dc.contributor.author |
Fu, S |
|
dc.contributor.author | Yun, K | |
dc.date.accessioned | 2022-01-06T04:06:45Z | |
dc.date.available | 2021-05-04 | |
dc.date.available | 2022-01-06T04:06:45Z | |
dc.date.issued | 2021-11-05 | |
dc.identifier.citation | Spectrochim Acta A Mol Biomol Spectrosc, 2021, 260, pp. 119927 | |
dc.identifier.issn | 1386-1425 | |
dc.identifier.issn | 1873-3557 | |
dc.identifier.uri | http://hdl.handle.net/10453/152743 | |
dc.description.abstract | Based on the specific interaction of Ag+ and cytosine-cytosine (C-C) base mismatch and using berberine (Ber) as the fluorescent probe and Exonuclease I (Exo I) as the background fluorescence reducing tool, a label-free Exo I-assisted fluorescence aptamer sensing platform was established for the detection of silver ions with high sensitivity and selectivity. Exo I reduced the fluorescence background of the Ber/Ag+-aptamer complex to a level similar to that of Ber itself in the absence of Ag+. After introducing Ag+ into the sensing system, it induces the aptamer rich in base C to form C-Ag+-C i-motif structure which are resistant to degradation mediated by Exo I. The concentration of Ber, Ag+-aptamer, Exo I and the temperature and reaction time for Exo I were all optimized. Under the optimal experimental conditions, the detection limit of Ag+ was 4.4 nM and the linear range was from 0.0059 μM to 235.48 μM with a coefficient of determination (R2) > 0.99. Moreover, the proposed strategy had been successfully applied to the detection of Ag+ in tap water and human serum with a good recovery ranging from 88.4% to 106.9%. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | Elsevier BV | |
dc.relation.ispartof | Spectrochim Acta A Mol Biomol Spectrosc | |
dc.relation.isbasedon | 10.1016/j.saa.2021.119927 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 0301 Analytical Chemistry, 0303 Macromolecular and Materials Chemistry, 0306 Physical Chemistry (incl. Structural) | |
dc.subject.classification | Chemical Physics | |
dc.subject.mesh | Aptamers, Nucleotide | |
dc.subject.mesh | Biosensing Techniques | |
dc.subject.mesh | Exodeoxyribonucleases | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Ions | |
dc.subject.mesh | Limit of Detection | |
dc.subject.mesh | Silver | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Ions | |
dc.subject.mesh | Silver | |
dc.subject.mesh | Exodeoxyribonucleases | |
dc.subject.mesh | Biosensing Techniques | |
dc.subject.mesh | Aptamers, Nucleotide | |
dc.subject.mesh | Limit of Detection | |
dc.title | A label-free Exonuclease I-assisted fluorescence aptasensor for highly selective and sensitive detection of silver ions. | |
dc.type | Journal Article | |
utslib.citation.volume | 260 | |
utslib.location.activity | England | |
utslib.for | 0301 Analytical Chemistry | |
utslib.for | 0303 Macromolecular and Materials Chemistry | |
utslib.for | 0306 Physical Chemistry (incl. Structural) | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - CFS - Centre for Forensic Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2022-01-06T04:06:44Z | |
pubs.publication-status | Published | |
pubs.volume | 260 |
Abstract:
Based on the specific interaction of Ag+ and cytosine-cytosine (C-C) base mismatch and using berberine (Ber) as the fluorescent probe and Exonuclease I (Exo I) as the background fluorescence reducing tool, a label-free Exo I-assisted fluorescence aptamer sensing platform was established for the detection of silver ions with high sensitivity and selectivity. Exo I reduced the fluorescence background of the Ber/Ag+-aptamer complex to a level similar to that of Ber itself in the absence of Ag+. After introducing Ag+ into the sensing system, it induces the aptamer rich in base C to form C-Ag+-C i-motif structure which are resistant to degradation mediated by Exo I. The concentration of Ber, Ag+-aptamer, Exo I and the temperature and reaction time for Exo I were all optimized. Under the optimal experimental conditions, the detection limit of Ag+ was 4.4 nM and the linear range was from 0.0059 μM to 235.48 μM with a coefficient of determination (R2) > 0.99. Moreover, the proposed strategy had been successfully applied to the detection of Ag+ in tap water and human serum with a good recovery ranging from 88.4% to 106.9%.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph