Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

Klont, F; Kwiatkowski, M; Faiz, A; van den Bosch, T; Pouwels, SD; Dekker, FJ; Ten Hacken, NHT; Horvatovich, P; Bischoff, R

Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

Klont, F Kwiatkowski, M Faiz, A

van den Bosch, T Pouwels, SD Dekker, FJ Ten Hacken, NHT Horvatovich, P Bischoff, R

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Publisher:: American Chemical Society (ACS)
Publication Type:: Journal Article
Citation:: J Proteome Res, 2021, 20, (11), pp. 5218-5221
Issue Date:: 2021-11-05

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Field	Value	Language
dc.contributor.author	Klont, F
dc.contributor.author	Kwiatkowski, M
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538
dc.contributor.author	van den Bosch, T
dc.contributor.author	Pouwels, SD
dc.contributor.author	Dekker, FJ
dc.contributor.author	Ten Hacken, NHT
dc.contributor.author	Horvatovich, P
dc.contributor.author	Bischoff, R
dc.date.accessioned	2022-01-08T02:41:03Z
dc.date.available	2022-01-08T02:41:03Z
dc.date.issued	2021-11-05
dc.identifier.citation	J Proteome Res, 2021, 20, (11), pp. 5218-5221
dc.identifier.issn	1535-3893
dc.identifier.issn	1535-3907
dc.identifier.uri	http://hdl.handle.net/10453/152826
dc.description.abstract	Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of a captured protein from the sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g., immunoassays) but hardly ever prior to liquid chromatography-mass spectrometry (LC-MS-)-based detection. Here, we describe the use of adsorptive microtiter plates for protein enrichment prior to LC-MS detection, and we discuss opportunities and challenges of corresponding workflows, based on examples of targeted (i.e., soluble receptor for advanced glycation end-products (sRAGE) in human serum) and discovery-based workflows (i.e., transcription factor p65 (NF-κB) in lysed murine RAW 264.7 macrophages and peptidyl-prolyl cis-trans isomerase FKBP5 (FKBP5) in lysed human A549 alveolar basal epithelial cells). Thereby, we aim to highlight the potential usefulness of adsorptive microtiter plates in affinity purification workflows prior to LC-MS detection, which could increase their usage in mass spectrometry-based protein research.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	American Chemical Society (ACS)
dc.relation.ispartof	J Proteome Res
dc.relation.isbasedon	10.1021/acs.jproteome.1c00623
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences
dc.subject.classification	Biochemistry & Molecular Biology
dc.title	Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.
dc.type	Journal Article
utslib.citation.volume	20
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-08T02:41:02Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	20
utslib.citation.issue	11

Abstract:

Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of a captured protein from the sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g., immunoassays) but hardly ever prior to liquid chromatography-mass spectrometry (LC-MS-)-based detection. Here, we describe the use of adsorptive microtiter plates for protein enrichment prior to LC-MS detection, and we discuss opportunities and challenges of corresponding workflows, based on examples of targeted (i.e., soluble receptor for advanced glycation end-products (sRAGE) in human serum) and discovery-based workflows (i.e., transcription factor p65 (NF-κB) in lysed murine RAW 264.7 macrophages and peptidyl-prolyl cis-trans isomerase FKBP5 (FKBP5) in lysed human A549 alveolar basal epithelial cells). Thereby, we aim to highlight the potential usefulness of adsorptive microtiter plates in affinity purification workflows prior to LC-MS detection, which could increase their usage in mass spectrometry-based protein research.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/152826