Dynamics of the Sydney rock oyster microbiota before and during a QX disease event

Nguyen, VK; King, WL; Siboni, N; Mahbub, KR; Rahman, MH; Jenkins, C; Dove, M; O'Connor, W; Seymour, JR; Labbate, M

Dynamics of the Sydney rock oyster microbiota before and during a QX disease event

Nguyen, VK King, WL Siboni, N

Mahbub, KR Rahman, MH Jenkins, C Dove, M O'Connor, W Seymour, JR Labbate, M

Permalink

Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Aquaculture (Amsterdam, Netherlands), 2021, 541, pp. Not-Available
Issue Date:: 2021-08

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Accepted versionAdobe PDF (1.43 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Nguyen, VK
dc.contributor.author	King, WL
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949
dc.contributor.author	Mahbub, KR
dc.contributor.author	Rahman, MH
dc.contributor.author	Jenkins, C
dc.contributor.author	Dove, M
dc.contributor.author	O'Connor, W
dc.contributor.author	Seymour, JR
dc.contributor.author	Labbate, M https://orcid.org/0000-0003-1428-3382
dc.date.accessioned	2022-01-12T04:11:57Z
dc.date.available	2022-01-12T04:11:57Z
dc.date.issued	2021-08
dc.identifier.citation	Aquaculture (Amsterdam, Netherlands), 2021, 541, pp. Not-Available
dc.identifier.issn	0044-8486
dc.identifier.issn	1873-5622
dc.identifier.uri	http://hdl.handle.net/10453/152964
dc.description.abstract	The Sydney rock oyster (SRO; Saccostrea glomerata) is the most intensively farmed oyster species in Australia however, Queensland unknown (QX) disease has resulted in substantial losses and impeded productivity. QX disease is caused by infection with the parasite Marteilia sydneyi, and like other diseases, outbreaks are driven by a series of complex environmental and host factors such as seasonality, seawater salinity and oyster genetics. A potential but understudied factor in QX disease is the SRO microbiota, which we sought to examine before and during a QX disease outbreak. Using 16S rRNA (V1 – V3 region) amplicon sequencing, we examined the microbiota of SROs deployed in an estuary where QX disease occurs, with sampling conducted fortnightly over 22 weeks. Marteilia sydneyi was detected in the SROs by PCR (QX-positive), 16 weeks after the first sampling event and sporonts were observed in the digestive gland two weeks later on. There were no apparent patterns observed between the microbiota of QX-positive SROs with and without digestive gland sporonts however, the microbiota of QX-positive SROs was significantly different from those sampled prior to detection of M. sydneyi and from those negative for M. sydneyi post detection. As a result, shifts in microbiota structure occurred before sporulation in the digestive gland and either before or shortly after pathogen colonisation. The microbiota shifts associated with QX-positive oysters were principally driven by a relative abundance increase of operational taxonomic units (OTUs) assigned to unclassified species of the Borrelia and Candidatus Hepatoplasma genera and a relative abundance decrease in an OTU assigned to an unclassified species of the Mycoplasma genus. Since Mycoplasma species are common microbiota features of SROs and other oysters, we propose that there may be an important ecological link between Mycoplasma species and the health state of SROs.
dc.format	Undetermined
dc.language	eng
dc.publisher	ELSEVIER
dc.relation	NSW Office of Environment and Heritage
dc.relation	NSW Department of Planning, Industry and Environment
dc.relation	http://purl.org/au-research/grants/arc/LP160101795
dc.relation.ispartof	Aquaculture (Amsterdam, Netherlands)
dc.relation.isbasedon	10.1016/j.aquaculture.2021.736821
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0608 Zoology, 0704 Fisheries Sciences
dc.subject.classification	Fisheries
dc.title	Dynamics of the Sydney rock oyster microbiota before and during a QX disease event
dc.type	Journal Article
utslib.citation.volume	541
utslib.for	0608 Zoology
utslib.for	0704 Fisheries Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-01-12T04:11:13Z
pubs.publication-status	Published
pubs.volume	541

Abstract:

The Sydney rock oyster (SRO; Saccostrea glomerata) is the most intensively farmed oyster species in Australia however, Queensland unknown (QX) disease has resulted in substantial losses and impeded productivity. QX disease is caused by infection with the parasite Marteilia sydneyi, and like other diseases, outbreaks are driven by a series of complex environmental and host factors such as seasonality, seawater salinity and oyster genetics. A potential but understudied factor in QX disease is the SRO microbiota, which we sought to examine before and during a QX disease outbreak. Using 16S rRNA (V1 – V3 region) amplicon sequencing, we examined the microbiota of SROs deployed in an estuary where QX disease occurs, with sampling conducted fortnightly over 22 weeks. Marteilia sydneyi was detected in the SROs by PCR (QX-positive), 16 weeks after the first sampling event and sporonts were observed in the digestive gland two weeks later on. There were no apparent patterns observed between the microbiota of QX-positive SROs with and without digestive gland sporonts however, the microbiota of QX-positive SROs was significantly different from those sampled prior to detection of M. sydneyi and from those negative for M. sydneyi post detection. As a result, shifts in microbiota structure occurred before sporulation in the digestive gland and either before or shortly after pathogen colonisation. The microbiota shifts associated with QX-positive oysters were principally driven by a relative abundance increase of operational taxonomic units (OTUs) assigned to unclassified species of the Borrelia and Candidatus Hepatoplasma genera and a relative abundance decrease in an OTU assigned to an unclassified species of the Mycoplasma genus. Since Mycoplasma species are common microbiota features of SROs and other oysters, we propose that there may be an important ecological link between Mycoplasma species and the health state of SROs.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/152964