Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes.

Hartmann, LM; Garcia, A; Deplazes, E; Cranfield, CG

Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes.

Hartmann, LM Garcia, A

Deplazes, E

Cranfield, CG

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Publisher:: Springer US
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2022, 2402, pp. 81-92
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Hartmann, LM
dc.contributor.author	Garcia, A https://orcid.org/0000-0002-1159-4567
dc.contributor.author	Deplazes, E https://orcid.org/0000-0003-2052-5536
dc.contributor.author	Cranfield, CG
dc.date.accessioned	2022-01-12T06:25:28Z
dc.date.available	2022-01-12T06:25:28Z
dc.date.issued	2022
dc.identifier.citation	Methods in Molecular Biology, 2022, 2402, pp. 81-92
dc.identifier.isbn	9781071618424
dc.identifier.uri	http://hdl.handle.net/10453/152994
dc.description.abstract	Swept frequency electrical impedance spectroscopy (EIS) can be used in conjunction with tethered bilayer lipid membranes to monitor the membrane permeability of ions in real-time (Deplazes et al. J Phys Chem Lett 11:6353-6358, 2020). Conductance readings, as determined by EIS, are a measure of the ability of ions to be transported across membranes. Recording the change in conductance as a function of cation concentration and a comparison between a range of cations permits conclusions to be made about the specificity of cation transport through pores. An estimate for upper pore size and cation selectivity of ion channels can be established using this method.
dc.format	Print
dc.language	en
dc.publisher	Springer US
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-1843-1_7
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.title	Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes.
dc.type	Chapter
utslib.citation.volume	2402
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/DVC (Teaching and Learning)
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-12T06:25:27Z
pubs.publication-status	Published
pubs.volume	2402

Abstract:

Swept frequency electrical impedance spectroscopy (EIS) can be used in conjunction with tethered bilayer lipid membranes to monitor the membrane permeability of ions in real-time (Deplazes et al. J Phys Chem Lett 11:6353-6358, 2020). Conductance readings, as determined by EIS, are a measure of the ability of ions to be transported across membranes. Recording the change in conductance as a function of cation concentration and a comparison between a range of cations permits conclusions to be made about the specificity of cation transport through pores. An estimate for upper pore size and cation selectivity of ion channels can be established using this method.

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http://hdl.handle.net/10453/152994