Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant.

Samardzic, K; Steele, JR; Violi, JP; Colville, A; Mitrovic, SM; Rodgers, KJ

Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant.

Samardzic, K

Steele, JR Violi, JP Colville, A

Mitrovic, SM Rodgers, KJ

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Publisher:: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication Type:: Journal Article
Citation:: Ecotoxicology and environmental safety, 2021, 208, pp. 111515-111515
Issue Date:: 2021-01-01

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Field	Value	Language
dc.contributor.author	Samardzic, K https://orcid.org/0000-0001-6177-5561
dc.contributor.author	Steele, JR
dc.contributor.author	Violi, JP
dc.contributor.author	Colville, A https://orcid.org/0000-0002-6543-2539
dc.contributor.author	Mitrovic, SM
dc.contributor.author	Rodgers, KJ
dc.date.accessioned	2022-01-13T06:14:40Z
dc.date.available	2020-10-13
dc.date.available	2022-01-13T06:14:40Z
dc.date.issued	2021-01-01
dc.identifier.citation	Ecotoxicology and environmental safety, 2021, 208, pp. 111515-111515
dc.identifier.issn	0147-6513
dc.identifier.issn	1090-2414
dc.identifier.uri	http://hdl.handle.net/10453/153067
dc.description.abstract	In order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root growth was significantly inhibited by both treatments. The content of derivatised free and protein-bound BMAA and DAB in seedlings was then analysed by LC-MS/MS. Both NPAAs were detected in free and protein-bound fractions with higher levels detected in free fractions. Compared to shoots, there was approximately tenfold more BMAA and DAB in alfalfa roots. These results suggest that NPAAs might be taken up into crop plants from contaminated irrigation water and enter the food chain. This may present an exposure pathway for NPAAs in humans.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartof	Ecotoxicology and environmental safety
dc.relation.isbasedon	10.1016/j.ecoenv.2020.111515
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	03 Chemical Sciences, 05 Environmental Sciences, 11 Medical and Health Sciences
dc.subject.classification	Strategic, Defence & Security Studies
dc.subject.mesh	Amino Acids, Diamino
dc.subject.mesh	Aminobutyrates
dc.subject.mesh	Bioaccumulation
dc.subject.mesh	Chromatography, Liquid
dc.subject.mesh	Crops, Agricultural
dc.subject.mesh	Cyanobacteria
dc.subject.mesh	Cyanobacteria Toxins
dc.subject.mesh	Humans
dc.subject.mesh	Isomerism
dc.subject.mesh	Neurotoxins
dc.subject.mesh	Seedlings
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Humans
dc.subject.mesh	Cyanobacteria
dc.subject.mesh	Crops, Agricultural
dc.subject.mesh	Amino Acids, Diamino
dc.subject.mesh	Neurotoxins
dc.subject.mesh	Chromatography, Liquid
dc.subject.mesh	Isomerism
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Aminobutyrates
dc.subject.mesh	Seedlings
dc.subject.mesh	Bioaccumulation
dc.subject.mesh	Cyanobacteria Toxins
dc.title	Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant.
dc.type	Journal Article
utslib.citation.volume	208
utslib.location.activity	Netherlands
utslib.for	03 Chemical Sciences
utslib.for	05 Environmental Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Provost
pubs.organisational-group	/University of Technology Sydney/Provost/Jumbunna
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-01-13T06:14:38Z
pubs.publication-status	Published
pubs.volume	208

Abstract:

In order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root growth was significantly inhibited by both treatments. The content of derivatised free and protein-bound BMAA and DAB in seedlings was then analysed by LC-MS/MS. Both NPAAs were detected in free and protein-bound fractions with higher levels detected in free fractions. Compared to shoots, there was approximately tenfold more BMAA and DAB in alfalfa roots. These results suggest that NPAAs might be taken up into crop plants from contaminated irrigation water and enter the food chain. This may present an exposure pathway for NPAAs in humans.

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http://hdl.handle.net/10453/153067