CD300a contributes to the resolution of articular inflammation triggered by MSU crystals by controlling neutrophil apoptosis.
- Publisher:
- WILEY
- Publication Type:
- Journal Article
- Citation:
- Immunology, 2021, 164, (2), pp. 305-317
- Issue Date:
- 2021-10
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Immunology - 2021 - Valiate - CD300a contributes to the resolution of articular inflammation triggered by MSU crystals by.pdf | Published version | 1.09 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Valiate, BVS | |
dc.contributor.author | Queiroz-Junior, CM | |
dc.contributor.author | Levi-Schaffer, F | |
dc.contributor.author | Galvão, I | |
dc.contributor.author | Teixeira, MM | |
dc.date.accessioned | 2022-01-18T22:15:57Z | |
dc.date.available | 2021-04-29 | |
dc.date.available | 2022-01-18T22:15:57Z | |
dc.date.issued | 2021-10 | |
dc.identifier.citation | Immunology, 2021, 164, (2), pp. 305-317 | |
dc.identifier.issn | 0019-2805 | |
dc.identifier.issn | 1365-2567 | |
dc.identifier.uri | http://hdl.handle.net/10453/153288 | |
dc.description.abstract | Gout is an inflammatory disease triggered by deposition of monosodium urate (MSU) crystals in the joints, resulting in high neutrophil influx and pain. Here, we studied the role of the inhibitory receptor CD300a in the resolution process in a murine model of gout. We found increased CD300a expression on neutrophils emigrated to the joint. When compared to WT mice, CD300a-/- mice had persistent neutrophil influx till 24 hr after MSU injection. This was associated with increased concentration of IL-1β and greater tissue damage in the joints of CD300a-/- mice. There was an increase in the percentage of apoptotic neutrophils in the synovial lavage of WT mice, as compared to CD300a-/- mice. This difference was reflected in the decline of efferocytic events in the synovial cavity of CD300a-/- mice 24 hr after MSU injection. A CD300a agonistic antibody was shown, for the first time, to increase apoptosis of human neutrophils, and this was associated with cleavage of caspase-8. In conclusion, our results reveal an important role of CD300a in the control of leucocyte infiltration, IL-1β production and caspase-8 cleavage in neutrophils, contributing to the resolution of inflammation triggered by MSU injection. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | WILEY | |
dc.relation.ispartof | Immunology | |
dc.relation.isbasedon | 10.1111/imm.13371 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 1107 Immunology, 1114 Paediatrics and Reproductive Medicine | |
dc.subject.classification | Immunology | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Apoptosis | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Gout | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Inflammation | |
dc.subject.mesh | Interleukin-1beta | |
dc.subject.mesh | Joints | |
dc.subject.mesh | Macrophages | |
dc.subject.mesh | Male | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Mice, Inbred BALB C | |
dc.subject.mesh | Neutrophils | |
dc.subject.mesh | Receptors, Immunologic | |
dc.subject.mesh | Uric Acid | |
dc.subject.mesh | Joints | |
dc.subject.mesh | Neutrophils | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Macrophages | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Mice, Inbred BALB C | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Gout | |
dc.subject.mesh | Inflammation | |
dc.subject.mesh | Uric Acid | |
dc.subject.mesh | Receptors, Immunologic | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Apoptosis | |
dc.subject.mesh | Male | |
dc.subject.mesh | Interleukin-1beta | |
dc.title | CD300a contributes to the resolution of articular inflammation triggered by MSU crystals by controlling neutrophil apoptosis. | |
dc.type | Journal Article | |
utslib.citation.volume | 164 | |
utslib.location.activity | England | |
utslib.for | 1107 Immunology | |
utslib.for | 1114 Paediatrics and Reproductive Medicine | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2022-01-18T22:15:56Z | |
pubs.issue | 2 | |
pubs.publication-status | Published | |
pubs.volume | 164 | |
utslib.citation.issue | 2 |
Abstract:
Gout is an inflammatory disease triggered by deposition of monosodium urate (MSU) crystals in the joints, resulting in high neutrophil influx and pain. Here, we studied the role of the inhibitory receptor CD300a in the resolution process in a murine model of gout. We found increased CD300a expression on neutrophils emigrated to the joint. When compared to WT mice, CD300a-/- mice had persistent neutrophil influx till 24 hr after MSU injection. This was associated with increased concentration of IL-1β and greater tissue damage in the joints of CD300a-/- mice. There was an increase in the percentage of apoptotic neutrophils in the synovial lavage of WT mice, as compared to CD300a-/- mice. This difference was reflected in the decline of efferocytic events in the synovial cavity of CD300a-/- mice 24 hr after MSU injection. A CD300a agonistic antibody was shown, for the first time, to increase apoptosis of human neutrophils, and this was associated with cleavage of caspase-8. In conclusion, our results reveal an important role of CD300a in the control of leucocyte infiltration, IL-1β production and caspase-8 cleavage in neutrophils, contributing to the resolution of inflammation triggered by MSU injection.
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