Chlamydia pecorum-Associated Sporadic Ovine Abortion.

Westermann, T; Jenkins, C; Onizawa, E; Gestier, S; McNally, J; Kirkland, P; Zhang, J; Bogema, D; Manning, LK; Walker, K; Pinczowski, P

Chlamydia pecorum-Associated Sporadic Ovine Abortion.

Westermann, T Jenkins, C Onizawa, E Gestier, S McNally, J Kirkland, P Zhang, J Bogema, D

Manning, LK Walker, K Pinczowski, P

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Publisher:: SAGE PUBLICATIONS INC
Publication Type:: Journal Article
Citation:: Vet Pathol, 2021, 58, (1), pp. 114-122
Issue Date:: 2021-01

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Field	Value	Language
dc.contributor.author	Westermann, T
dc.contributor.author	Jenkins, C
dc.contributor.author	Onizawa, E
dc.contributor.author	Gestier, S
dc.contributor.author	McNally, J
dc.contributor.author	Kirkland, P
dc.contributor.author	Zhang, J
dc.contributor.author	Bogema, D https://orcid.org/0000-0001-7013-7326
dc.contributor.author	Manning, LK
dc.contributor.author	Walker, K
dc.contributor.author	Pinczowski, P
dc.date.accessioned	2022-01-23T00:03:12Z
dc.date.available	2022-01-23T00:03:12Z
dc.date.issued	2021-01
dc.identifier.citation	Vet Pathol, 2021, 58, (1), pp. 114-122
dc.identifier.issn	0300-9858
dc.identifier.issn	1544-2217
dc.identifier.uri	http://hdl.handle.net/10453/153446
dc.description.abstract	Despite previous detection of Chlamydia pecorum in sporadic ovine abortions, published descriptions of naturally occurring infections with fetoplacental lesions are lacking. This report provides the first descriptions of severe necrosuppurative chorionitis with vasculitis, and fetal pyelonephritis and enteritis in late-term abortions of maiden ewes. Chlamydial infection was detected using a Chlamydia genus-specific qPCR (quantitative polymerase chain reaction) on tissue extracts from 3 fetuses. C. pecorum was identified using a targeted qPCR assay, which also determined infectious load within fetal tissues. The presence of viable C. pecorum in fetal samples was confirmed by cell culture. Multilocus sequence typing (MLST) data indicated that the C. pecorum strains from each fetus were identical and of sequence type (ST) 23. Chlamydia sp. immunohistochemistry showed strong positive immunolabeling of fetoplacental lesions. Other infectious abortigenic agents were excluded with specific testing. This report confirms C. pecorum as a likely cause of ovine abortion and provides the first descriptions of associated fetoplacental lesions in naturally infected sheep.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SAGE PUBLICATIONS INC
dc.relation.ispartof	Vet Pathol
dc.relation.isbasedon	10.1177/0300985820967451
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0704 Fisheries Sciences, 0707 Veterinary Sciences
dc.subject.classification	Veterinary Sciences
dc.subject.mesh	Animals
dc.subject.mesh	Chlamydia
dc.subject.mesh	Chlamydia Infections
dc.subject.mesh	Female
dc.subject.mesh	Multilocus Sequence Typing
dc.subject.mesh	Pregnancy
dc.subject.mesh	Sheep
dc.subject.mesh	Sheep Diseases
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Chlamydia
dc.subject.mesh	Chlamydia Infections
dc.subject.mesh	Sheep Diseases
dc.subject.mesh	Pregnancy
dc.subject.mesh	Female
dc.subject.mesh	Multilocus Sequence Typing
dc.title	Chlamydia pecorum-Associated Sporadic Ovine Abortion.
dc.type	Journal Article
utslib.citation.volume	58
utslib.location.activity	United States
utslib.for	0704 Fisheries Sciences
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-23T00:03:10Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	58
utslib.citation.issue	1

Abstract:

Despite previous detection of Chlamydia pecorum in sporadic ovine abortions, published descriptions of naturally occurring infections with fetoplacental lesions are lacking. This report provides the first descriptions of severe necrosuppurative chorionitis with vasculitis, and fetal pyelonephritis and enteritis in late-term abortions of maiden ewes. Chlamydial infection was detected using a Chlamydia genus-specific qPCR (quantitative polymerase chain reaction) on tissue extracts from 3 fetuses. C. pecorum was identified using a targeted qPCR assay, which also determined infectious load within fetal tissues. The presence of viable C. pecorum in fetal samples was confirmed by cell culture. Multilocus sequence typing (MLST) data indicated that the C. pecorum strains from each fetus were identical and of sequence type (ST) 23. Chlamydia sp. immunohistochemistry showed strong positive immunolabeling of fetoplacental lesions. Other infectious abortigenic agents were excluded with specific testing. This report confirms C. pecorum as a likely cause of ovine abortion and provides the first descriptions of associated fetoplacental lesions in naturally infected sheep.

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http://hdl.handle.net/10453/153446