Evidence that DOPA-derivatives are generated after L-DOPA incorporation into proteins by mammalian cells

Thompson, AM; Dunlop, RA; Dean, RT; Rodgers, KJ

Evidence that DOPA-derivatives are generated after L-DOPA incorporation into proteins by mammalian cells

Thompson, AM Dunlop, RA Dean, RT Rodgers, KJ

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Publication Type:: Journal Article
Citation:: Journal of Adhesion, 2009, 85 (9), pp. 561 - 575
Issue Date:: 2009-09-21

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Field	Value	Language
dc.contributor.author	Thompson, AM	en_US
dc.contributor.author	Dunlop, RA	en_US
dc.contributor.author	Dean, RT	en_US
dc.contributor.author	Rodgers, KJ https://orcid.org/0000-0002-3627-9651	en_US
dc.date.issued	2009-09-21	en_US
dc.identifier.citation	Journal of Adhesion, 2009, 85 (9), pp. 561 - 575	en_US
dc.identifier.issn	0021-8464	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15361
dc.description.abstract	The adhesive and cohesive properties of the amino acid L-3,4-dihydroxyphenylalanine (DOPA) have been widely explored as a potential material for adhesion, based, among other things, on the biological system of blue mussel extracellular byssal threads and foot proteins. Proteins containing DOPA are generated within mammalian cells by oxidation of tyrosine residues during periods of oxidative stress. By generating proteins containing DOPA, in vitro, through the (mis)incorporation of DOPA during protein synthesis, we are able examine the role and fate of DOPA-containing proteins in mammalian cells. We demonstrate a decrease in catabolism of long half-life cell proteins and an increase in cellular autofluorescence when DOPA is present in cell proteins. We provide evidence for the formation of DOPA derivatives which can be detected in proteins after 14C-DOPA incorporation by HPLC analysis. Additionally, we demonstrate that the cells upregulate the expression of genes required to handle damaged proteins and protein aggregates under these conditions. Substantial evidence for DOPA derivatives and cross-linking has previously been shown in extracellular blue mussel byssal threads; here we provide evidence for cell-associated DOPA derivatives in mammalian cells.	en_US
dc.relation.ispartof	Journal of Adhesion	en_US
dc.relation.isbasedon	10.1080/00218460902996747	en_US
dc.subject.classification	Polymers	en_US
dc.title	Evidence that DOPA-derivatives are generated after L-DOPA incorporation into proteins by mammalian cells	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	85	en_US
utslib.for	0912 Materials Engineering	en_US
dc.location.activity	ISI:000269604000001	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	85	en_US

Abstract:

The adhesive and cohesive properties of the amino acid L-3,4-dihydroxyphenylalanine (DOPA) have been widely explored as a potential material for adhesion, based, among other things, on the biological system of blue mussel extracellular byssal threads and foot proteins. Proteins containing DOPA are generated within mammalian cells by oxidation of tyrosine residues during periods of oxidative stress. By generating proteins containing DOPA, in vitro, through the (mis)incorporation of DOPA during protein synthesis, we are able examine the role and fate of DOPA-containing proteins in mammalian cells. We demonstrate a decrease in catabolism of long half-life cell proteins and an increase in cellular autofluorescence when DOPA is present in cell proteins. We provide evidence for the formation of DOPA derivatives which can be detected in proteins after 14C-DOPA incorporation by HPLC analysis. Additionally, we demonstrate that the cells upregulate the expression of genes required to handle damaged proteins and protein aggregates under these conditions. Substantial evidence for DOPA derivatives and cross-linking has previously been shown in extracellular blue mussel byssal threads; here we provide evidence for cell-associated DOPA derivatives in mammalian cells.

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http://hdl.handle.net/10453/15361