Comparative transcriptome analysis of inner blood-retinal barrier and blood-brain barrier in rats.

Li, Y; Faiz, A; Moshage, H; Schubert, R; Schilling, L; Kamps, JA

Comparative transcriptome analysis of inner blood-retinal barrier and blood-brain barrier in rats.

Li, Y Faiz, A

Moshage, H Schubert, R Schilling, L Kamps, JA

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Publisher:: NATURE RESEARCH
Publication Type:: Journal Article
Citation:: Sci Rep, 2021, 11, (1), pp. 12151
Issue Date:: 2021-06-09

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Field	Value	Language
dc.contributor.author	Li, Y
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538
dc.contributor.author	Moshage, H
dc.contributor.author	Schubert, R
dc.contributor.author	Schilling, L
dc.contributor.author	Kamps, JA
dc.date.accessioned	2022-01-27T23:10:27Z
dc.date.available	2021-05-13
dc.date.available	2022-01-27T23:10:27Z
dc.date.issued	2021-06-09
dc.identifier.citation	Sci Rep, 2021, 11, (1), pp. 12151
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/153680
dc.description.abstract	Although retinal microvessels (RMVs) and brain microvessels (BMVs) are closely related in their developmental and share similar blood-neural barriers, studies have reported markedly different responses to stressors such as diabetes. Therefore, we hypothesized that RMVs and BMVs will display substantial differences in gene expression levels even though they are of the same embryological origin. In this study, both RMVs and BMVs were mechanically isolated from rats. Full retinal and brain tissue samples (RT, BT) were collected for comparisons. Total RNA extracted from these four groups were processed on Affymetrix rat 2.0 microarray Chips. The transcriptional profiles of these tissues were then analyzed. In the present paper we looked at differentially expressed genes (DEGs) in RMVs (against RT) and BMVs (against BT) using a rather conservative threshold value of ≥ ± twofold change and a false discovery rate corrected for multiple comparisons (p < 0.05). In RMVs a total of 1559 DEGs were found, of which 1004 genes were higher expressed in RMVs than in RT. Moreover, 4244 DEGs between BMVs and BT were identified, of which 1956 genes were ≥ twofold enriched in BMVs. Using these DEGs, we comprehensively analyzed the actual expression levels and highlighted their involvement in critical functional structures in RMVs and BMVs, such as junctional complex, transporters and signaling pathways. Our work provides for the first time the transcriptional profiles of rat RMVs and BMVs. These results may help to understand why retina and brain microvasculature show different susceptibilities to stressors, and they might even provide new insight for pharmacological interventions.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE RESEARCH
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/s41598-021-91584-7
dc.rights	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2021
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.mesh	Animals
dc.subject.mesh	Biological Transport
dc.subject.mesh	Blood-Brain Barrier
dc.subject.mesh	Blood-Retinal Barrier
dc.subject.mesh	Brain
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Male
dc.subject.mesh	Microvessels
dc.subject.mesh	Rats
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Retina
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Transcriptome
dc.subject.mesh	Blood-Brain Barrier
dc.subject.mesh	Blood-Retinal Barrier
dc.subject.mesh	Brain
dc.subject.mesh	Retina
dc.subject.mesh	Animals
dc.subject.mesh	Rats
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Biological Transport
dc.subject.mesh	Male
dc.subject.mesh	Microvessels
dc.subject.mesh	Transcriptome
dc.title	Comparative transcriptome analysis of inner blood-retinal barrier and blood-brain barrier in rats.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	recently_added	*
dc.date.updated	2022-01-27T23:10:22Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	11
utslib.citation.issue	1

Abstract:

Although retinal microvessels (RMVs) and brain microvessels (BMVs) are closely related in their developmental and share similar blood-neural barriers, studies have reported markedly different responses to stressors such as diabetes. Therefore, we hypothesized that RMVs and BMVs will display substantial differences in gene expression levels even though they are of the same embryological origin. In this study, both RMVs and BMVs were mechanically isolated from rats. Full retinal and brain tissue samples (RT, BT) were collected for comparisons. Total RNA extracted from these four groups were processed on Affymetrix rat 2.0 microarray Chips. The transcriptional profiles of these tissues were then analyzed. In the present paper we looked at differentially expressed genes (DEGs) in RMVs (against RT) and BMVs (against BT) using a rather conservative threshold value of ≥ ± twofold change and a false discovery rate corrected for multiple comparisons (p < 0.05). In RMVs a total of 1559 DEGs were found, of which 1004 genes were higher expressed in RMVs than in RT. Moreover, 4244 DEGs between BMVs and BT were identified, of which 1956 genes were ≥ twofold enriched in BMVs. Using these DEGs, we comprehensively analyzed the actual expression levels and highlighted their involvement in critical functional structures in RMVs and BMVs, such as junctional complex, transporters and signaling pathways. Our work provides for the first time the transcriptional profiles of rat RMVs and BMVs. These results may help to understand why retina and brain microvasculature show different susceptibilities to stressors, and they might even provide new insight for pharmacological interventions.

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http://hdl.handle.net/10453/153680