Cord blood group 2 innate lymphoid cells are associated with lung function at 6 weeks of age.

Martins Costa Gomes, G; de Gouveia Belinelo, P; Starkey, MR; Murphy, VE; Hansbro, PM; Sly, PD; Robinson, PD; Karmaus, W; Gibson, PG; Mattes, J; Collison, AM

Cord blood group 2 innate lymphoid cells are associated with lung function at 6 weeks of age.

Martins Costa Gomes, G de Gouveia Belinelo, P Starkey, MR Murphy, VE Hansbro, PM Sly, PD Robinson, PD Karmaus, W Gibson, PG Mattes, J Collison, AM

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Clin Transl Immunology, 2021, 10, (7), pp. e1296
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Martins Costa Gomes, G
dc.contributor.author	de Gouveia Belinelo, P
dc.contributor.author	Starkey, MR
dc.contributor.author	Murphy, VE
dc.contributor.author	Hansbro, PM
dc.contributor.author	Sly, PD
dc.contributor.author	Robinson, PD
dc.contributor.author	Karmaus, W
dc.contributor.author	Gibson, PG
dc.contributor.author	Mattes, J
dc.contributor.author	Collison, AM
dc.date.accessioned	2022-01-28T19:13:18Z
dc.date.available	2021-05-21
dc.date.available	2022-01-28T19:13:18Z
dc.date.issued	2021
dc.identifier.citation	Clin Transl Immunology, 2021, 10, (7), pp. e1296
dc.identifier.issn	2050-0068
dc.identifier.issn	2050-0068
dc.identifier.uri	http://hdl.handle.net/10453/153773
dc.description.abstract	Objective: Offspring born to mothers with asthma in pregnancy are known to have lower lung function which tracks with age. Human group 2 innate lymphoid cells (ILC2) accumulate in foetal lungs, at 10-fold higher levels compared to adult lungs. However, there are no data on foetal ILC2 numbers and the association with respiratory health outcomes such as lung function in early life. We aimed to investigate cord blood immune cell populations from babies born to mothers with asthma in pregnancy. Methods: Cord blood from babies born to asthmatic mothers was collected, and cells were stained in whole cord blood. Analyses were done using traditional gating approaches and computational methodologies (t-distributed stochastic neighbour embedding and PhenoGraph algorithms). At 6 weeks of age, the time to peak tidal expiratory flow as a percentage of total expiratory flow time (tPTEF/tE%) was determined as well as Lung Clearance Index (LCI), during quiet natural sleep. Results: Of 110 eligible infants (March 2017 to November 2019), 91 were successfully immunophenotyped (82.7%). Lung function was attempted in 61 infants (67.0%), and 43 of those infants (70.5% of attempted) had technically acceptable tPTEF/tE% measurements. Thirty-four infants (55.7% of attempted) had acceptable LCI measurements. Foetal ILC2 numbers with increased expression of chemoattractant receptor-homologous molecule (CRTh2), characterised by two distinct analysis methodologies, were associated with poorer infant lung function at 6 weeks of age." Conclusion: Foetal immune responses may be a surrogate variable for or directly influence lung function outcomes in early life.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	WILEY
dc.relation	http://purl.org/au-research/grants/nhmrc/1175134
dc.relation.ispartof	Clin Transl Immunology
dc.relation.isbasedon	10.1002/cti2.1296
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1107 Immunology, 1115 Pharmacology and Pharmaceutical Sciences
dc.title	Cord blood group 2 innate lymphoid cells are associated with lung function at 6 weeks of age.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Australia
utslib.for	1107 Immunology
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-01-28T19:13:14Z
pubs.issue	7
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	7

Abstract:

Objective: Offspring born to mothers with asthma in pregnancy are known to have lower lung function which tracks with age. Human group 2 innate lymphoid cells (ILC2) accumulate in foetal lungs, at 10-fold higher levels compared to adult lungs. However, there are no data on foetal ILC2 numbers and the association with respiratory health outcomes such as lung function in early life. We aimed to investigate cord blood immune cell populations from babies born to mothers with asthma in pregnancy. Methods: Cord blood from babies born to asthmatic mothers was collected, and cells were stained in whole cord blood. Analyses were done using traditional gating approaches and computational methodologies (t-distributed stochastic neighbour embedding and PhenoGraph algorithms). At 6 weeks of age, the time to peak tidal expiratory flow as a percentage of total expiratory flow time (tPTEF/tE%) was determined as well as Lung Clearance Index (LCI), during quiet natural sleep. Results: Of 110 eligible infants (March 2017 to November 2019), 91 were successfully immunophenotyped (82.7%). Lung function was attempted in 61 infants (67.0%), and 43 of those infants (70.5% of attempted) had technically acceptable tPTEF/tE% measurements. Thirty-four infants (55.7% of attempted) had acceptable LCI measurements. Foetal ILC2 numbers with increased expression of chemoattractant receptor-homologous molecule (CRTh2), characterised by two distinct analysis methodologies, were associated with poorer infant lung function at 6 weeks of age." Conclusion: Foetal immune responses may be a surrogate variable for or directly influence lung function outcomes in early life.

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http://hdl.handle.net/10453/153773