Extensively resistant Acinetobacter baumannii isolate RCH52 carries several resistance genes derived from an IncC plasmid.

Ambrose, SJ; Hamidian, M; Hall, RM

Extensively resistant Acinetobacter baumannii isolate RCH52 carries several resistance genes derived from an IncC plasmid.

Ambrose, SJ Hamidian, M

Hall, RM

Permalink

Publisher:: Oxford University Press (OUP)
Publication Type:: Journal Article
Citation:: J Antimicrob Chemother, 2022
Issue Date:: 2022-01-18

Closed Access

	Filename	Description	Size
	2022_Ambrose-Hamidian-Hall-JAC_dkab473.pdf	Published version	425.04 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Ambrose, SJ
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261
dc.contributor.author	Hall, RM
dc.date.accessioned	2022-01-31T21:59:17Z
dc.date.available	2021-11-26
dc.date.available	2022-01-31T21:59:17Z
dc.date.issued	2022-01-18
dc.identifier.citation	J Antimicrob Chemother, 2022
dc.identifier.issn	0305-7453
dc.identifier.issn	1460-2091
dc.identifier.uri	http://hdl.handle.net/10453/153986
dc.description.abstract	OBJECTIVES: To identify the origins of resistance in a sporadic extensively resistant Acinetobacter baumannii isolate. METHODS: The complete genome of RCH52 was determined by combining available Illumina short reads with MinION (Oxford Nanopore) long reads using Unicycler. Bioinformatic searches were used to identify features of interest. RESULTS: The complete genome of RCH52 revealed an unusual chromosomal region containing all of the antibiotic resistance genes, except tet39, which is in a plasmid. A 129 585 bp segment was bounded by inversely oriented copies of ISAba1 and included two groups of resistance genes separated by the large segment of the backbone of type 1 IncC plasmids that lies between the ARI-A and ARI-B resistance islands but does not include the replication region. The ISAba1-bounded segment was located in a novel integrative element that had integrated into the chromosomal thyA gene but provided a replacement thyA gene. Several resistance genes are derived from either the ARI-A or the ARI-B resistance islands found in IncC plasmids that have been brought together by an IS26-mediated deletion of the original plasmid. This non-replicating circular molecule (or translocatable unit) has been incorporated into a smaller ISAba1-bounded unit that includes oxa23 in Tn2008B via homologous recombination between sul2-CR2-floR segments found in both. CONCLUSIONS: The plasmids shared by most Gram-negative pathogens, including the broad host range IncC plasmids, have not been detected in Acinetobacter species. However, it seems likely that they can conjugate into members of this genus and contribute pre-existing clusters of antibiotic resistance genes.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Oxford University Press (OUP)
dc.relation.ispartof	J Antimicrob Chemother
dc.relation.isbasedon	10.1093/jac/dkab473
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology, 1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Microbiology
dc.title	Extensively resistant Acinetobacter baumannii isolate RCH52 carries several resistance genes derived from an IncC plasmid.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-31T21:59:16Z
pubs.publication-status	Published online

Abstract:

OBJECTIVES: To identify the origins of resistance in a sporadic extensively resistant Acinetobacter baumannii isolate. METHODS: The complete genome of RCH52 was determined by combining available Illumina short reads with MinION (Oxford Nanopore) long reads using Unicycler. Bioinformatic searches were used to identify features of interest. RESULTS: The complete genome of RCH52 revealed an unusual chromosomal region containing all of the antibiotic resistance genes, except tet39, which is in a plasmid. A 129 585 bp segment was bounded by inversely oriented copies of ISAba1 and included two groups of resistance genes separated by the large segment of the backbone of type 1 IncC plasmids that lies between the ARI-A and ARI-B resistance islands but does not include the replication region. The ISAba1-bounded segment was located in a novel integrative element that had integrated into the chromosomal thyA gene but provided a replacement thyA gene. Several resistance genes are derived from either the ARI-A or the ARI-B resistance islands found in IncC plasmids that have been brought together by an IS26-mediated deletion of the original plasmid. This non-replicating circular molecule (or translocatable unit) has been incorporated into a smaller ISAba1-bounded unit that includes oxa23 in Tn2008B via homologous recombination between sul2-CR2-floR segments found in both. CONCLUSIONS: The plasmids shared by most Gram-negative pathogens, including the broad host range IncC plasmids, have not been detected in Acinetobacter species. However, it seems likely that they can conjugate into members of this genus and contribute pre-existing clusters of antibiotic resistance genes.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/153986