Preservation of and DNA Extraction from Muscle Tissue.

McNevin, D

Preservation of and DNA Extraction from Muscle Tissue.

McNevin, D

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Publisher:: Springer New York
Publication Type:: Journal Article
Citation:: Methods Mol Biol, 2016, 1420, pp. 43-53
Issue Date:: 2016

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	Filename	Description	Size
	McNevin (2016) DNA preservatives in Goodwin (ed) Forensic DNA typing protocols.pdf	Published version	106.84 kB	Adobe PDF	View/Open
	2016_Bookmatter_ForensicDNATypingProtocols.pdf	Supporting information	302.24 kB	Adobe PDF	View/Open

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Field	Value	Language
dc.contributor.author	McNevin, D https://orcid.org/0000-0003-1665-3367
dc.date.accessioned	2022-01-31T22:37:05Z
dc.date.available	2022-01-31T22:37:05Z
dc.date.issued	2016
dc.identifier.citation	Methods Mol Biol, 2016, 1420, pp. 43-53
dc.identifier.isbn	9781493935956
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/154002
dc.description.abstract	As well as protecting DNA for subsequent analysis, tissue preservation methods ideally should be safe, readily available, and easy to transport at relatively low cost. Formalin (formaldehyde solution), used extensively to preserve medical and museum specimens, irreparably damages DNA. We have found four tissue preservatives (solid salt, salt-saturated dimethyl sulfoxide (DMSO)-EDTA solution, ethanol solution, and ethanol-EDTA solution) that preserved muscle tissue at 35 °C for up to 1 month: full short tandem repeat (STR) profiles were obtained after preservation. In addition, salt-saturated DMSO-EDTA solution yielded full STR profiles from aliquots of the liquid preservative surrounding muscle tissue.
dc.format	Print
dc.language	eng
dc.publisher	Springer New York
dc.relation.ispartof	Methods Mol Biol
dc.relation.isbasedon	10.1007/978-1-4939-3597-0_4
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	DNA
dc.subject.mesh	DNA Fingerprinting
dc.subject.mesh	Forensic Anthropology
dc.subject.mesh	Forensic Genetics
dc.subject.mesh	Humans
dc.subject.mesh	Microsatellite Repeats
dc.subject.mesh	Muscles
dc.subject.mesh	Reference Standards
dc.subject.mesh	Muscles
dc.subject.mesh	Humans
dc.subject.mesh	DNA
dc.subject.mesh	DNA Fingerprinting
dc.subject.mesh	Microsatellite Repeats
dc.subject.mesh	Reference Standards
dc.subject.mesh	Forensic Anthropology
dc.subject.mesh	Forensic Genetics
dc.title	Preservation of and DNA Extraction from Muscle Tissue.
dc.type	Journal Article
utslib.citation.volume	1420
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-31T22:37:00Z
pubs.publication-status	Published
pubs.volume	1420

Abstract:

As well as protecting DNA for subsequent analysis, tissue preservation methods ideally should be safe, readily available, and easy to transport at relatively low cost. Formalin (formaldehyde solution), used extensively to preserve medical and museum specimens, irreparably damages DNA. We have found four tissue preservatives (solid salt, salt-saturated dimethyl sulfoxide (DMSO)-EDTA solution, ethanol solution, and ethanol-EDTA solution) that preserved muscle tissue at 35 °C for up to 1 month: full short tandem repeat (STR) profiles were obtained after preservation. In addition, salt-saturated DMSO-EDTA solution yielded full STR profiles from aliquots of the liquid preservative surrounding muscle tissue.

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http://hdl.handle.net/10453/154002