Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype.

Ariyama, N; Agüero, B; Valdés, V; Berrios, F; Bucarey, S; Mor, S; Brito, B; Neira, V

Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype.

Ariyama, N Agüero, B Valdés, V Berrios, F Bucarey, S Mor, S Brito, B Neira, V

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Publisher:: Frontiers Media
Publication Type:: Journal Article
Citation:: Frontiers in Veterinary Science, 2021, 8, pp. 1-6
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Ariyama, N
dc.contributor.author	Agüero, B
dc.contributor.author	Valdés, V
dc.contributor.author	Berrios, F
dc.contributor.author	Bucarey, S
dc.contributor.author	Mor, S
dc.contributor.author	Brito, B
dc.contributor.author	Neira, V
dc.date.accessioned	2022-02-03T04:35:28Z
dc.date.available	2021-11-23
dc.date.available	2022-02-03T04:35:28Z
dc.date.issued	2021
dc.identifier.citation	Frontiers in Veterinary Science, 2021, 8, pp. 1-6
dc.identifier.issn	2297-1769
dc.identifier.issn	2297-1769
dc.identifier.uri	http://hdl.handle.net/10453/154167
dc.description.abstract	Porcine Circovirus 2 (PCV2) can cause multiple clinical conditions known as porcine circovirus-associated diseases (PCVAD). Before the wide availability of PCV2 vaccines, PCVAD resulted in significant losses to the global swine industry. PCV2's rapid evolutionary dynamics are comparable to single-stranded RNA viruses. Thus, shifts in the dominance and distribution of different genotypes may frequently occur, resulting in the emergence and spread of varying PCV2 genotypes and recombinant strains in swine. This study aims at identifying the PCV2 genotypes currently circulating in Chile. Seven hundred thirty-eight samples were obtained from 21 swine farms between 2020 and 2021. The samples were tested using PCR for species detection and genotyping. Sequencing and phylogenetic analyses were conducted in selected samples. PCV2 was detected in 26.9% of the PCR reactions and 67% of the sampled farms. The genotypes were determined in nine farms, PCV2a in one farm, PCV2b in four, and PCV2d in five, with PCV2b and PCV2d co-circulating in one farm. The phylogenetic analysis of twelve ORF2 sequences obtained (PCV2a = 5; PCV2b = 4; PCV2d = 3) showed a PCV2a Chilean strains monophyletic cluster; closely related to Chilean viruses collected in 2012 and 2013. Of the three different PCV2b sequenced viruses, two viruses were close to the root of the PCV2b group, whereas the remaining one grouped with a South Korean virus. PCV2d sequences were closely related to Asian viruses. A previously reported PCV2a/PCV2d recombinant strain was not detected in this study. Our results suggest the emergence and potential shift to PCV2d genotype in Chilean farms.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Frontiers Media
dc.relation.ispartof	Frontiers in Veterinary Science
dc.relation.isbasedon	10.3389/fvets.2021.789491
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0707 Veterinary Sciences
dc.title	Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	Switzerland
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-02-03T04:35:24Z
pubs.publication-status	Published
pubs.volume	8

Abstract:

Porcine Circovirus 2 (PCV2) can cause multiple clinical conditions known as porcine circovirus-associated diseases (PCVAD). Before the wide availability of PCV2 vaccines, PCVAD resulted in significant losses to the global swine industry. PCV2's rapid evolutionary dynamics are comparable to single-stranded RNA viruses. Thus, shifts in the dominance and distribution of different genotypes may frequently occur, resulting in the emergence and spread of varying PCV2 genotypes and recombinant strains in swine. This study aims at identifying the PCV2 genotypes currently circulating in Chile. Seven hundred thirty-eight samples were obtained from 21 swine farms between 2020 and 2021. The samples were tested using PCR for species detection and genotyping. Sequencing and phylogenetic analyses were conducted in selected samples. PCV2 was detected in 26.9% of the PCR reactions and 67% of the sampled farms. The genotypes were determined in nine farms, PCV2a in one farm, PCV2b in four, and PCV2d in five, with PCV2b and PCV2d co-circulating in one farm. The phylogenetic analysis of twelve ORF2 sequences obtained (PCV2a = 5; PCV2b = 4; PCV2d = 3) showed a PCV2a Chilean strains monophyletic cluster; closely related to Chilean viruses collected in 2012 and 2013. Of the three different PCV2b sequenced viruses, two viruses were close to the root of the PCV2b group, whereas the remaining one grouped with a South Korean virus. PCV2d sequences were closely related to Asian viruses. A previously reported PCV2a/PCV2d recombinant strain was not detected in this study. Our results suggest the emergence and potential shift to PCV2d genotype in Chilean farms.

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http://hdl.handle.net/10453/154167