High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay.

Sutherland, DL; Burke, J; Ralph, PJ

High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay.

Sutherland, DL Burke, J Ralph, PJ

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: N Biotechnol, 2021, 65, pp. 61-68
Issue Date:: 2021-11-25

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Field	Value	Language
dc.contributor.author	Sutherland, DL
dc.contributor.author	Burke, J
dc.contributor.author	Ralph, PJ
dc.date.accessioned	2022-02-06T04:47:58Z
dc.date.available	2021-08-05
dc.date.available	2022-02-06T04:47:58Z
dc.date.issued	2021-11-25
dc.identifier.citation	N Biotechnol, 2021, 65, pp. 61-68
dc.identifier.issn	1871-6784
dc.identifier.issn	1876-4347
dc.identifier.uri	http://hdl.handle.net/10453/154219
dc.description.abstract	Microalgae produce a broad range of organic compounds that are increasingly being recognised for their value in novel product production and biotechnological applications. Most microalgae are photoautotrophic, but some are capable of either mixotrophy or heterotrophy. Reported enhanced biomass yields or contrasting metabolite profiles compared to autotrophic growth improve the economics of large-scale production of microalgae, which currently limits industrial applications. Here, the potential of a high-throughput method for the rapid screening of microalgal metabolism was assessed against 95 different carbon sources, using the cost-effective Biolog plate. Of the 5 microalgae tested, Desmodesmus communis (30 carbon sources) and Chlorella vulgaris (19 carbon sources) had the highest number of positive responses to carbon sources, whereas Chlorella sorokiniana had the most negative (toxic) response to the various carbon sources (77 carbon sources). Comparison of Biolog plate results with traditional culture techniques showed good agreement. Species with a high number of positive responses on the Biolog plate exhibited the highest biomass yield under heterotrophic conditions, whilst those with low number of positive responses exhibited the highest biomass yield under autotrophic conditions, using traditional culturing techniques. While the use of these plates is limited to obtaining axenic lines of microalgal species, the method provided a high-throughput assessment of carbon source metabolism, without the expense of undertaking large, laborious traditional culturing assessments. Such high-throughput assessments can be regarded as useful tools for progressing species selection, metabolic capacity and optimal culture conditions for microalgal biotechnology applications.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER
dc.relation.ispartof	N Biotechnol
dc.relation.isbasedon	10.1016/j.nbt.2021.08.001
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 10 Technology
dc.subject.classification	Biotechnology
dc.subject.mesh	Biomass
dc.subject.mesh	Carbon
dc.subject.mesh	Chlorella vulgaris
dc.subject.mesh	Heterotrophic Processes
dc.subject.mesh	High-Throughput Screening Assays
dc.subject.mesh	Microalgae
dc.subject.mesh	Chlorella vulgaris
dc.subject.mesh	Carbon
dc.subject.mesh	Biomass
dc.subject.mesh	Heterotrophic Processes
dc.subject.mesh	High-Throughput Screening Assays
dc.subject.mesh	Microalgae
dc.title	High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay.
dc.type	Journal Article
utslib.citation.volume	65
utslib.location.activity	Netherlands
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access	*
dc.date.updated	2022-02-06T04:47:56Z
pubs.publication-status	Published
pubs.volume	65

Abstract:

Microalgae produce a broad range of organic compounds that are increasingly being recognised for their value in novel product production and biotechnological applications. Most microalgae are photoautotrophic, but some are capable of either mixotrophy or heterotrophy. Reported enhanced biomass yields or contrasting metabolite profiles compared to autotrophic growth improve the economics of large-scale production of microalgae, which currently limits industrial applications. Here, the potential of a high-throughput method for the rapid screening of microalgal metabolism was assessed against 95 different carbon sources, using the cost-effective Biolog plate. Of the 5 microalgae tested, Desmodesmus communis (30 carbon sources) and Chlorella vulgaris (19 carbon sources) had the highest number of positive responses to carbon sources, whereas Chlorella sorokiniana had the most negative (toxic) response to the various carbon sources (77 carbon sources). Comparison of Biolog plate results with traditional culture techniques showed good agreement. Species with a high number of positive responses on the Biolog plate exhibited the highest biomass yield under heterotrophic conditions, whilst those with low number of positive responses exhibited the highest biomass yield under autotrophic conditions, using traditional culturing techniques. While the use of these plates is limited to obtaining axenic lines of microalgal species, the method provided a high-throughput assessment of carbon source metabolism, without the expense of undertaking large, laborious traditional culturing assessments. Such high-throughput assessments can be regarded as useful tools for progressing species selection, metabolic capacity and optimal culture conditions for microalgal biotechnology applications.

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http://hdl.handle.net/10453/154219