On-Site Detection of Carcinoembryonic Antigen in Human Serum

Mahmoudi, T; Pourhassan-Moghaddam, M; Shirdel, B; Baradaran, B; Morales-Narváez, E; Golmohammadi, H

On-Site Detection of Carcinoembryonic Antigen in Human Serum

Mahmoudi, T Pourhassan-Moghaddam, M Shirdel, B Baradaran, B Morales-Narváez, E Golmohammadi, H

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Biosensors, 2021, 11, (10), pp. 392
Issue Date:: 2021-10-14

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Field	Value	Language
dc.contributor.author	Mahmoudi, T
dc.contributor.author	Pourhassan-Moghaddam, M
dc.contributor.author	Shirdel, B
dc.contributor.author	Baradaran, B
dc.contributor.author	Morales-Narváez, E
dc.contributor.author	Golmohammadi, H
dc.date.accessioned	2022-02-08T02:06:18Z
dc.date.available	2022-02-08T02:06:18Z
dc.date.issued	2021-10-14
dc.identifier.citation	Biosensors, 2021, 11, (10), pp. 392
dc.identifier.issn	2079-6374
dc.identifier.issn	2079-6374
dc.identifier.uri	http://hdl.handle.net/10453/154265
dc.description.abstract	<jats:p>Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation.ispartof	Biosensors
dc.relation.isbasedon	10.3390/bios11100392
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology
dc.title	On-Site Detection of Carcinoembryonic Antigen in Human Serum
dc.type	Journal Article
utslib.citation.volume	11
utslib.for	0301 Analytical Chemistry
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-02-08T02:06:16Z
pubs.issue	10
pubs.publication-status	Published online
pubs.volume	11
utslib.citation.issue	10

Abstract:

Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.

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http://hdl.handle.net/10453/154265