Monitoring fitness and productivity in cyanobacteria batch cultures

Zavřel, T; Schoffman, H; Lukeš, M; Fedorko, J; Keren, N; Červený, J

Monitoring fitness and productivity in cyanobacteria batch cultures

Zavřel, T Schoffman, H Lukeš, M Fedorko, J Keren, N Červený, J

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Algal Research, 2021, 56, pp. 1-15
Issue Date:: 2021-06-01

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Field	Value	Language
dc.contributor.author	Zavřel, T
dc.contributor.author	Schoffman, H
dc.contributor.author	Lukeš, M
dc.contributor.author	Fedorko, J
dc.contributor.author	Keren, N
dc.contributor.author	Červený, J
dc.date.accessioned	2022-02-10T05:24:27Z
dc.date.available	2022-02-10T05:24:27Z
dc.date.issued	2021-06-01
dc.identifier.citation	Algal Research, 2021, 56, pp. 1-15
dc.identifier.issn	2211-9264
dc.identifier.issn	2211-9264
dc.identifier.uri	http://hdl.handle.net/10453/154377
dc.description.abstract	Cyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest.
dc.language	English
dc.publisher	Elsevier
dc.relation.ispartof	Algal Research
dc.relation.isbasedon	10.1016/j.algal.2021.102328
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0607 Plant Biology, 0904 Chemical Engineering, 1003 Industrial Biotechnology
dc.title	Monitoring fitness and productivity in cyanobacteria batch cultures
dc.type	Journal Article
utslib.citation.volume	56
utslib.for	0607 Plant Biology
utslib.for	0904 Chemical Engineering
utslib.for	1003 Industrial Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-02-10T05:24:24Z
pubs.publication-status	Published
pubs.volume	56

Abstract:

Cyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest.

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http://hdl.handle.net/10453/154377