Field |
Value |
Language |
dc.contributor.author |
Abril, D |
|
dc.contributor.author |
Vergara, E |
|
dc.contributor.author |
Palacios, D |
|
dc.contributor.author |
Leal, AL |
|
dc.contributor.author |
Marquez-Ortiz, RA |
|
dc.contributor.author |
Madroñero, J |
|
dc.contributor.author |
Rozo, ZLC |
|
dc.contributor.author |
De La Rosa, Z |
|
dc.contributor.author |
Nieto, CA |
|
dc.contributor.author |
Vanegas, N |
|
dc.contributor.author |
Cortés, JA |
|
dc.contributor.author |
Escobar-Perez, J |
|
dc.date.accessioned |
2022-02-24T04:28:38Z |
|
dc.date.available |
2022-02-24T04:28:38Z |
|
dc.date.issued |
2021-11-01 |
|
dc.identifier.citation |
Scientific Reports, 2021, 11, (1), pp. 21409 |
|
dc.identifier.issn |
2045-2322 |
|
dc.identifier.issn |
2045-2322 |
|
dc.identifier.uri |
http://hdl.handle.net/10453/154831
|
|
dc.description.abstract |
<jats:title>Abstract</jats:title><jats:p>Resistance to carbapenems in <jats:italic>Klebsiella</jats:italic><jats:italic>pneumoniae</jats:italic> has been mostly related with the worldwide dissemination of KPC, largely due to the pandemic clones belonging to the complex clonal (CC) 258. To unravel <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub> post-endemic clinical impact, here we describe clinical characteristics of 68 patients from a high complexity hospital, and the molecular and genetic characteristics of their 139 <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub>—<jats:italic>K.</jats:italic><jats:italic>pneumoniae</jats:italic> (KPC-<jats:italic>Kp</jats:italic>) isolates. Of the 26 patients that presented relapses or reinfections, 16 had changes in the resistance profiles of the isolates recovered from the recurrent episodes. In respect to the genetic diversity of KPC-<jats:italic>Kp</jats:italic> isolates, PFGE revealed 45 different clonal complexes (CC). MLST for 12 representative clones showed ST258 was present in the most frequent CC (23.0%), however, remaining 11 representative clones belonged to non-CC258 STs (77.0%). Interestingly, 16 patients presented within-patient genetic diversity of KPC-<jats:italic>Kp</jats:italic> clones. In one of these, three unrelated KPC-<jats:italic>Kp</jats:italic> clones (ST258, ST504, and ST846) and a <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub>—<jats:italic>K.</jats:italic><jats:italic>variicola</jats:italic> isolate (ST182) were identified. For this patient, complete genome sequence of one representative isolate of each clone was determined. In <jats:italic>K.</jats:italic><jats:italic>pneumoniae</jats:italic> isolates <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub> was mobilized by two Tn<jats:italic>3</jats:italic>-like unrelated platforms: Tn<jats:italic>4401b</jats:italic> (ST258) and Tn<jats:italic>6454</jats:italic> (ST504 and ST846)<jats:italic>,</jats:italic> a new NTE<jats:sub>KPC-</jats:sub>IIe transposon for first time characterized also determined in the <jats:italic>K.</jats:italic><jats:italic>variicola</jats:italic> isolate of this study. Genome analysis showed these transposons were harbored in different unrelated but previously reported plasmids and in the chromosome of a <jats:italic>K.</jats:italic><jats:italic>pneumoniae</jats:italic> (for Tn<jats:italic>4401b</jats:italic>). In conclusion, in the <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub> post-endemic dissemination in Colombia, different KPC-<jats:italic>Kp</jats:italic> clones (mostly non-CC258) have emerged due to integration of the single <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub> gene in new genetic platforms. This work also shows the intra-patient resistant and genetic diversity of KPC-<jats:italic>Kp</jats:italic> isolates. This circulation dynamic could impact the effectiveness of long-term treatments.</jats:p> |
|
dc.language |
en |
|
dc.publisher |
Springer Science and Business Media LLC |
|
dc.relation.ispartof |
Scientific Reports |
|
dc.relation.isbasedon |
10.1038/s41598-021-00887-2 |
|
dc.rights |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or
format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the
Creative Commons licence, and indicate if changes were made. The images or other third party material in this
article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the
material. If material is not included in the article’s Creative Commons licence and your intended use is not
permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2021 |
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
dc.title |
Within patient genetic diversity of blaKPC harboring Klebsiellapneumoniae in a Colombian hospital and identification of a new NTEKPC platform |
|
dc.type |
Journal Article |
|
utslib.citation.volume |
11 |
|
pubs.organisational-group |
/University of Technology Sydney |
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science |
|
utslib.copyright.status |
open_access |
* |
dc.date.updated |
2022-02-24T04:28:09Z |
|
pubs.issue |
1 |
|
pubs.publication-status |
Published |
|
pubs.volume |
11 |
|
utslib.citation.issue |
1 |
|