Photoinduced Photosensitizer-Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV.

Sadraeian, M; da Cruz, EF; Boyle, RW; Bahou, C; Chudasama, V; Janini, LMR; Diaz, RS; Guimarães, FEG

Photoinduced Photosensitizer-Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV.

Sadraeian, M

da Cruz, EF Boyle, RW Bahou, C Chudasama, V Janini, LMR Diaz, RS Guimarães, FEG

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS Omega, 2021, 6, (25), pp. 16524-16534
Issue Date:: 2021-06-29

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Field	Value	Language
dc.contributor.author	Sadraeian, M https://orcid.org/0000-0002-7384-2247
dc.contributor.author	da Cruz, EF
dc.contributor.author	Boyle, RW
dc.contributor.author	Bahou, C
dc.contributor.author	Chudasama, V
dc.contributor.author	Janini, LMR
dc.contributor.author	Diaz, RS
dc.contributor.author	Guimarães, FEG
dc.date.accessioned	2022-03-13T21:55:32Z
dc.date.available	2021-05-27
dc.date.available	2022-03-13T21:55:32Z
dc.date.issued	2021-06-29
dc.identifier.citation	ACS Omega, 2021, 6, (25), pp. 16524-16534
dc.identifier.issn	2470-1343
dc.identifier.issn	2470-1343
dc.identifier.uri	http://hdl.handle.net/10453/155186
dc.description.abstract	HIV-infected cells persist for decades in patients administered with antiretroviral therapy (ART). Meanwhile, an alarming surge in drug-resistant HIV viruses has been occurring. Addressing these issues, we propose the application of photoimmunotherapy (PIT) against not only HIV Env-expressing cells but also HIV. Previously, we showed that a human anti-gp41 antibody (7B2) conjugated to cationic or anionic photosensitizers (PSs) could specifically target and kill the HIV Env-expressing cells. Here, our photolysis studies revealed that the binding of photoimmunoconjugates (PICs) on the membrane of HIV Env-expressing cells is sufficient to induce necrotic cell death due to physical damage to the membrane by singlet oxygen, which is independent of the type of PSs. This finding persuaded us to study the virus photoinactivation of PICs using two HIV-1 strains, X4 HIV-1 NL4-3 and JR-CSF virus. We observed that the PICs could destroy the viral strains, probably via physical damage on the HIV envelope. In conclusion, we report the application of PIT as a possible dual-tool for HIV immunotherapy and ART by killing HIV-expressing cells and cell-free HIV, respectively.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	ACS Omega
dc.relation.isbasedon	10.1021/acsomega.1c01721
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0904 Chemical Engineering, 0912 Materials Engineering
dc.title	Photoinduced Photosensitizer-Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV.
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	United States
utslib.for	0904 Chemical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-03-13T21:55:25Z
pubs.issue	25
pubs.publication-status	Published online
pubs.volume	6
utslib.citation.issue	25

Abstract:

HIV-infected cells persist for decades in patients administered with antiretroviral therapy (ART). Meanwhile, an alarming surge in drug-resistant HIV viruses has been occurring. Addressing these issues, we propose the application of photoimmunotherapy (PIT) against not only HIV Env-expressing cells but also HIV. Previously, we showed that a human anti-gp41 antibody (7B2) conjugated to cationic or anionic photosensitizers (PSs) could specifically target and kill the HIV Env-expressing cells. Here, our photolysis studies revealed that the binding of photoimmunoconjugates (PICs) on the membrane of HIV Env-expressing cells is sufficient to induce necrotic cell death due to physical damage to the membrane by singlet oxygen, which is independent of the type of PSs. This finding persuaded us to study the virus photoinactivation of PICs using two HIV-1 strains, X4 HIV-1 NL4-3 and JR-CSF virus. We observed that the PICs could destroy the viral strains, probably via physical damage on the HIV envelope. In conclusion, we report the application of PIT as a possible dual-tool for HIV immunotherapy and ART by killing HIV-expressing cells and cell-free HIV, respectively.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/155186