Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma.

Behary, J; Amorim, N; Jiang, X-T; Raposo, A; Gong, L; McGovern, E; Ibrahim, R; Chu, F; Stephens, C; Jebeili, H; Fragomeli, V; Koay, YC; Jackson, M; O'Sullivan, J; Weltman, M; McCaughan, G; El-Omar, E; Zekry, A

Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma.

Behary, J Amorim, N Jiang, X-T Raposo, A Gong, L McGovern, E Ibrahim, R Chu, F Stephens, C Jebeili, H Fragomeli, V Koay, YC Jackson, M O'Sullivan, J Weltman, M McCaughan, G El-Omar, E Zekry, A

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Publisher:: Nature Research
Publication Type:: Journal Article
Citation:: Nature Communications, 2021, 12, (1), pp. 1-14
Issue Date:: 2021-01-08

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Field	Value	Language
dc.contributor.author	Behary, J
dc.contributor.author	Amorim, N
dc.contributor.author	Jiang, X-T
dc.contributor.author	Raposo, A
dc.contributor.author	Gong, L
dc.contributor.author	McGovern, E
dc.contributor.author	Ibrahim, R
dc.contributor.author	Chu, F
dc.contributor.author	Stephens, C
dc.contributor.author	Jebeili, H
dc.contributor.author	Fragomeli, V
dc.contributor.author	Koay, YC
dc.contributor.author	Jackson, M
dc.contributor.author	O'Sullivan, J
dc.contributor.author	Weltman, M
dc.contributor.author	McCaughan, G
dc.contributor.author	El-Omar, E
dc.contributor.author	Zekry, A
dc.date.accessioned	2022-03-15T00:55:09Z
dc.date.available	2020-11-23
dc.date.available	2022-03-15T00:55:09Z
dc.date.issued	2021-01-08
dc.identifier.citation	Nature Communications, 2021, 12, (1), pp. 1-14
dc.identifier.issn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10453/155231
dc.description.abstract	The gut microbiota is reported to modulate the immune response in hepatocellular carcinoma (HCC). Here, we employ metagenomic and metabolomic studies to characterise gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) related cirrhosis, with or without HCC, and evaluate its effect on the peripheral immune response in an ex vivo model. We find that dysbiosis characterises the microbiota of patients with NAFLD-cirrhosis, with compositional and functional shifts occurring with HCC development. Gene function of the microbiota in NAFLD-HCC supports short chain fatty acid production, and this is confirmed by metabolomic studies. Ex vivo studies show that bacterial extracts from the NAFLD-HCC microbiota, but not from the control groups, elicit a T cell immunosuppressive phenotype, characterised by expansion of regulatory T cells and attenuation of CD8 + T cells. Our study suggest that the gut microbiota in NAFLD-HCC is characterised by a distinctive microbiome/metabolomic profile, and can modulate the peripheral immune response.
dc.format	Electronic
dc.language	eng
dc.publisher	Nature Research
dc.relation.ispartof	Nature Communications
dc.relation.isbasedon	10.1038/s41467-020-20422-7
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Aged
dc.subject.mesh	Bacteria
dc.subject.mesh	Carcinoma, Hepatocellular
dc.subject.mesh	CD8-Positive T-Lymphocytes
dc.subject.mesh	Cytokines
dc.subject.mesh	Dietary Fiber
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Fatty Acids, Volatile
dc.subject.mesh	Feces
dc.subject.mesh	Female
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Humans
dc.subject.mesh	Immunity
dc.subject.mesh	Liver
dc.subject.mesh	Liver Cirrhosis
dc.subject.mesh	Liver Neoplasms
dc.subject.mesh	Male
dc.subject.mesh	Metabolomics
dc.subject.mesh	Metagenomics
dc.subject.mesh	Middle Aged
dc.subject.mesh	Non-alcoholic Fatty Liver Disease
dc.subject.mesh	Phenotype
dc.subject.mesh	Aged
dc.subject.mesh	Bacteria
dc.subject.mesh	CD8-Positive T-Lymphocytes
dc.subject.mesh	Carcinoma, Hepatocellular
dc.subject.mesh	Cytokines
dc.subject.mesh	Dietary Fiber
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Fatty Acids, Volatile
dc.subject.mesh	Feces
dc.subject.mesh	Female
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Humans
dc.subject.mesh	Immunity
dc.subject.mesh	Liver
dc.subject.mesh	Liver Cirrhosis
dc.subject.mesh	Liver Neoplasms
dc.subject.mesh	Male
dc.subject.mesh	Metabolomics
dc.subject.mesh	Metagenomics
dc.subject.mesh	Middle Aged
dc.subject.mesh	Non-alcoholic Fatty Liver Disease
dc.subject.mesh	Phenotype
dc.subject.mesh	Liver
dc.subject.mesh	CD8-Positive T-Lymphocytes
dc.subject.mesh	Feces
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Carcinoma, Hepatocellular
dc.subject.mesh	Liver Neoplasms
dc.subject.mesh	Liver Cirrhosis
dc.subject.mesh	Fatty Acids, Volatile
dc.subject.mesh	Cytokines
dc.subject.mesh	Immunity
dc.subject.mesh	Phenotype
dc.subject.mesh	Dietary Fiber
dc.subject.mesh	Aged
dc.subject.mesh	Middle Aged
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Metabolomics
dc.subject.mesh	Metagenomics
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Non-alcoholic Fatty Liver Disease
dc.subject.mesh	Gastrointestinal Microbiome
dc.title	Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-15T00:54:59Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	12
utslib.citation.issue	1

Abstract:

The gut microbiota is reported to modulate the immune response in hepatocellular carcinoma (HCC). Here, we employ metagenomic and metabolomic studies to characterise gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) related cirrhosis, with or without HCC, and evaluate its effect on the peripheral immune response in an ex vivo model. We find that dysbiosis characterises the microbiota of patients with NAFLD-cirrhosis, with compositional and functional shifts occurring with HCC development. Gene function of the microbiota in NAFLD-HCC supports short chain fatty acid production, and this is confirmed by metabolomic studies. Ex vivo studies show that bacterial extracts from the NAFLD-HCC microbiota, but not from the control groups, elicit a T cell immunosuppressive phenotype, characterised by expansion of regulatory T cells and attenuation of CD8 + T cells. Our study suggest that the gut microbiota in NAFLD-HCC is characterised by a distinctive microbiome/metabolomic profile, and can modulate the peripheral immune response.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/155231