A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies

Stelzer-Braid, S; Wong, B; Robertson, P; Lynch, GW; Laurie, K; Shaw, R; Barr, I; Selleck, PW; Baleriola, C; Escott, R; Katsoulotos, G; Rawlinson, WD

A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies

Wong, B Robertson, P Lynch, GW Laurie, K Shaw, R Barr, I Selleck, PW Baleriola, C Escott, R Katsoulotos, G Rawlinson, WD

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Publication Type:: Journal Article
Citation:: Journal of Clinical Virology, 2008, 43 (2), pp. 241 - 243
Issue Date:: 2008-10-01

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Field	Value	Language
dc.contributor.author	Stelzer-Braid, S https://orcid.org/0000-0001-6037-9305	en_US
dc.contributor.author	Wong, B	en_US
dc.contributor.author	Robertson, P	en_US
dc.contributor.author	Lynch, GW	en_US
dc.contributor.author	Laurie, K	en_US
dc.contributor.author	Shaw, R	en_US
dc.contributor.author	Barr, I	en_US
dc.contributor.author	Selleck, PW	en_US
dc.contributor.author	Baleriola, C	en_US
dc.contributor.author	Escott, R	en_US
dc.contributor.author	Katsoulotos, G	en_US
dc.contributor.author	Rawlinson, WD	en_US
dc.date.available	2008-06-24	en_US
dc.date.issued	2008-10-01	en_US
dc.identifier.citation	Journal of Clinical Virology, 2008, 43 (2), pp. 241 - 243	en_US
dc.identifier.issn	1386-6532	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15548
dc.description.abstract	Background and objectives: Commercial serological assays to determine influenza A H5N1 infection are available, although the accuracy and reproducibility of these are not reported in detail. This study aimed to assess the validity of a commercial ELISA H5 hemagglutinin (HA) antibody kit. Study design: A commercial ELISA for detection of antibodies towards influenza A H5 HA was evaluated using human sera from vaccinated individuals. The ELISA was used to screen 304 sera with elevated influenza A complement fixation titres collected between the period 1995-2007. Results and conclusions: The ELISA was found to be accurate for sera with high levels of anti-H5 antibodies, and would be useful in clinical settings where a rapid result is required. Thirteen of the stored sera were positive using the ELISA, but were confirmed as negative for H5N1 exposure using further serological tests. Absorption studies suggested that antibodies towards seasonal H3N2 and H1N1 influenza may cross-react with H5 antigen, giving false positive results with the ELISA. © 2008 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Journal of Clinical Virology	en_US
dc.relation.isbasedon	10.1016/j.jcv.2008.06.012	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Hemagglutinin Glycoproteins, Influenza Virus	en_US
dc.subject.mesh	Antibodies, Viral	en_US
dc.subject.mesh	Reagent Kits, Diagnostic	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Complement Fixation Tests	en_US
dc.subject.mesh	Hemagglutination Inhibition Tests	en_US
dc.subject.mesh	Cross Reactions	en_US
dc.subject.mesh	Adolescent	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Aged, 80 and over	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Influenza, Human	en_US
dc.subject.mesh	Influenza A Virus, H3N2 Subtype	en_US
dc.subject.mesh	Influenza A Virus, H5N1 Subtype	en_US
dc.subject.mesh	Influenza A Virus, H1N1 Subtype	en_US
dc.subject.mesh	Young Adult	en_US
dc.title	A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	43	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	43	en_US

Abstract:

Background and objectives: Commercial serological assays to determine influenza A H5N1 infection are available, although the accuracy and reproducibility of these are not reported in detail. This study aimed to assess the validity of a commercial ELISA H5 hemagglutinin (HA) antibody kit. Study design: A commercial ELISA for detection of antibodies towards influenza A H5 HA was evaluated using human sera from vaccinated individuals. The ELISA was used to screen 304 sera with elevated influenza A complement fixation titres collected between the period 1995-2007. Results and conclusions: The ELISA was found to be accurate for sera with high levels of anti-H5 antibodies, and would be useful in clinical settings where a rapid result is required. Thirteen of the stored sera were positive using the ELISA, but were confirmed as negative for H5N1 exposure using further serological tests. Absorption studies suggested that antibodies towards seasonal H3N2 and H1N1 influenza may cross-react with H5 antigen, giving false positive results with the ELISA. © 2008 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/15548