Cardiac health in the reduced uterine perfusion pressure rat model of preeclampsia

Richard, C; Chhor, M; Glenn Ong, ZH; Bubb, K; McClements, L

Cardiac health in the reduced uterine perfusion pressure rat model of preeclampsia

Richard, C Chhor, M Glenn Ong, ZH Bubb, K McClements, L

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Publisher:: Wiley
Publication Type:: Conference Proceeding
Citation:: Journal of Paediatrics and Child Health, 2020, 56, (S1)
Issue Date:: 2020-01-01

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Field	Value	Language
dc.contributor.author	Richard, C
dc.contributor.author	Chhor, M
dc.contributor.author	Glenn Ong, ZH
dc.contributor.author	Bubb, K
dc.contributor.author	McClements, L https://orcid.org/0000-0002-4911-1014
dc.date	2021-02-10
dc.date.accessioned	2022-03-24T23:07:38Z
dc.date.available	2022-03-24T23:07:38Z
dc.date.issued	2020-01-01
dc.identifier.citation	Journal of Paediatrics and Child Health, 2020, 56, (S1)
dc.identifier.issn	1034-4810
dc.identifier.uri	http://hdl.handle.net/10453/155526
dc.description.abstract	Background: Preeclampsia (PE) is a life-threatening disorder without treatment options except delivery of the baby and the placenta, often pre-term. PE increases the risk of subsequent cardiovascular complications in both mothers and offspring. In this study, our aim was to investigate the associated cardiac health in the reduced uterine perfusion pressure (RUPP) rat model of PE. Methods: The RUPP model was induced in pregnant rats (GD14) by applying silver clips around aorta and uterine arteries reducing the blood flow to the uterus by ~40%. On GD19, echocardiography was performed and blood pressure measured. At the end of the experiment, maternal organs were harvested and processed for downstream analyses. Results: Whilst RUPP rats had increased systolic pressure (sham 113 ± 1 vs RUPP 125 ± 2 mmHg, n=8, P<0.001), diastolic blood pressure was substantially augmented and in the hypertensive range (sham 88 ± 2 vs RUPP 102 ± 2 mmHg, n=8, P<0.0001). Left ventricular mass was greater in RUPP females (n=6-7, P=0.03), however wet heart weight to body weight ratio did not reach statistical significance (sham 0.30 ± 0.01 vs RUPP 0.33 ± 0.01, n=6, P=0.07). Left ventricular systolic function remained unaffected. Also increased expression of anti-angiogenic FKBPL mRNA (0.85 ± 0.15, n=6 vs 1.82 ± 0.27, n=6) was observed in the RUPP hearts. Conclusions: Despite no difference in cardiac systolic function in the RUPP model, early signs of restricted angiogenesis and greater augmentation of diastolic pressure suggest that diastolic dysfunction may be occurring, which will be investigated in future studies.
dc.language	en
dc.publisher	Wiley
dc.relation.ispartof	Journal of Paediatrics and Child Health
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1103 Clinical Sciences, 1114 Paediatrics and Reproductive Medicine, 1117 Public Health and Health Services
dc.subject.classification	Pediatrics
dc.title	Cardiac health in the reduced uterine perfusion pressure rat model of preeclampsia
dc.type	Conference Proceeding
utslib.citation.volume	56
utslib.location.activity	Sydney
utslib.for	1103 Clinical Sciences
utslib.for	1114 Paediatrics and Reproductive Medicine
utslib.for	1117 Public Health and Health Services
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-24T23:07:36Z
pubs.finish-date	2021-02-13
pubs.issue	S1
pubs.publication-status	Published online
pubs.start-date	2021-02-10
pubs.volume	56
utslib.citation.issue	S1

Abstract:

Background: Preeclampsia (PE) is a life-threatening disorder without treatment options except delivery of the baby and the placenta, often pre-term. PE increases the risk of subsequent cardiovascular complications in both mothers and offspring. In this study, our aim was to investigate the associated cardiac health in the reduced uterine perfusion pressure (RUPP) rat model of PE. Methods: The RUPP model was induced in pregnant rats (GD14) by applying silver clips around aorta and uterine arteries reducing the blood flow to the uterus by ~40%. On GD19, echocardiography was performed and blood pressure measured. At the end of the experiment, maternal organs were harvested and processed for downstream analyses. Results: Whilst RUPP rats had increased systolic pressure (sham 113 ± 1 vs RUPP 125 ± 2 mmHg, n=8, P<0.001), diastolic blood pressure was substantially augmented and in the hypertensive range (sham 88 ± 2 vs RUPP 102 ± 2 mmHg, n=8, P<0.0001). Left ventricular mass was greater in RUPP females (n=6-7, P=0.03), however wet heart weight to body weight ratio did not reach statistical significance (sham 0.30 ± 0.01 vs RUPP 0.33 ± 0.01, n=6, P=0.07). Left ventricular systolic function remained unaffected. Also increased expression of anti-angiogenic FKBPL mRNA (0.85 ± 0.15, n=6 vs 1.82 ± 0.27, n=6) was observed in the RUPP hearts. Conclusions: Despite no difference in cardiac systolic function in the RUPP model, early signs of restricted angiogenesis and greater augmentation of diastolic pressure suggest that diastolic dysfunction may be occurring, which will be investigated in future studies.

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http://hdl.handle.net/10453/155526