Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells.
Polikarpov, D
Liang, L
Care, A
Sunna, A
Campbell, D
Walsh, B
Balalaeva, I
Zvyagin, A
Gillatt, D
Guryev, E
- Publisher:
- MDPI
- Publication Type:
- Journal Article
- Citation:
- Biomolecules, 2019, 9, (12)
- Issue Date:
- 2019-12-03
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Polikarpov, D | |
dc.contributor.author | Liang, L | |
dc.contributor.author |
Care, A https://orcid.org/0000-0002-0035-7961 |
|
dc.contributor.author | Sunna, A | |
dc.contributor.author | Campbell, D | |
dc.contributor.author | Walsh, B | |
dc.contributor.author | Balalaeva, I | |
dc.contributor.author | Zvyagin, A | |
dc.contributor.author | Gillatt, D | |
dc.contributor.author | Guryev, E | |
dc.date.accessioned | 2022-03-25T04:19:18Z | |
dc.date.available | 2019-12-02 | |
dc.date.available | 2022-03-25T04:19:18Z | |
dc.date.issued | 2019-12-03 | |
dc.identifier.citation | Biomolecules, 2019, 9, (12) | |
dc.identifier.issn | 2218-273X | |
dc.identifier.issn | 2218-273X | |
dc.identifier.uri | http://hdl.handle.net/10453/155552 | |
dc.description.abstract | Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.relation.ispartof | Biomolecules | |
dc.relation.isbasedon | 10.3390/biom9120820 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0601 Biochemistry and Cell Biology | |
dc.subject.mesh | Antibodies, Monoclonal | |
dc.subject.mesh | Carcinoma, Transitional Cell | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Glypicans | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Luminescent Agents | |
dc.subject.mesh | Nanoparticles | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.subject.mesh | Silicon Dioxide | |
dc.subject.mesh | Spectrometry, Fluorescence | |
dc.subject.mesh | Urinary Bladder Neoplasms | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Carcinoma, Transitional Cell | |
dc.subject.mesh | Silicon Dioxide | |
dc.subject.mesh | Antibodies, Monoclonal | |
dc.subject.mesh | Luminescent Agents | |
dc.subject.mesh | Spectrometry, Fluorescence | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.subject.mesh | Urinary Bladder Neoplasms | |
dc.subject.mesh | Glypicans | |
dc.subject.mesh | Nanoparticles | |
dc.title | Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells. | |
dc.type | Journal Article | |
utslib.citation.volume | 9 | |
utslib.location.activity | Switzerland | |
utslib.for | 0601 Biochemistry and Cell Biology | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2022-03-25T04:19:12Z | |
pubs.issue | 12 | |
pubs.publication-status | Published online | |
pubs.volume | 9 | |
utslib.citation.issue | 12 |
Abstract:
Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.
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