Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells.

Polikarpov, D; Liang, L; Care, A; Sunna, A; Campbell, D; Walsh, B; Balalaeva, I; Zvyagin, A; Gillatt, D; Guryev, E

Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells.

Polikarpov, D Liang, L Care, A

Sunna, A Campbell, D Walsh, B Balalaeva, I Zvyagin, A Gillatt, D Guryev, E

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Biomolecules, 2019, 9, (12)
Issue Date:: 2019-12-03

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Field	Value	Language
dc.contributor.author	Polikarpov, D
dc.contributor.author	Liang, L
dc.contributor.author	Care, A https://orcid.org/0000-0002-0035-7961
dc.contributor.author	Sunna, A
dc.contributor.author	Campbell, D
dc.contributor.author	Walsh, B
dc.contributor.author	Balalaeva, I
dc.contributor.author	Zvyagin, A
dc.contributor.author	Gillatt, D
dc.contributor.author	Guryev, E
dc.date.accessioned	2022-03-25T04:19:18Z
dc.date.available	2019-12-02
dc.date.available	2022-03-25T04:19:18Z
dc.date.issued	2019-12-03
dc.identifier.citation	Biomolecules, 2019, 9, (12)
dc.identifier.issn	2218-273X
dc.identifier.issn	2218-273X
dc.identifier.uri	http://hdl.handle.net/10453/155552
dc.description.abstract	Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Biomolecules
dc.relation.isbasedon	10.3390/biom9120820
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.subject.mesh	Antibodies, Monoclonal
dc.subject.mesh	Carcinoma, Transitional Cell
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Glypicans
dc.subject.mesh	Humans
dc.subject.mesh	Luminescent Agents
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Silicon Dioxide
dc.subject.mesh	Spectrometry, Fluorescence
dc.subject.mesh	Urinary Bladder Neoplasms
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Humans
dc.subject.mesh	Carcinoma, Transitional Cell
dc.subject.mesh	Silicon Dioxide
dc.subject.mesh	Antibodies, Monoclonal
dc.subject.mesh	Luminescent Agents
dc.subject.mesh	Spectrometry, Fluorescence
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Urinary Bladder Neoplasms
dc.subject.mesh	Glypicans
dc.subject.mesh	Nanoparticles
dc.title	Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells.
dc.type	Journal Article
utslib.citation.volume	9
utslib.location.activity	Switzerland
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-03-25T04:19:12Z
pubs.issue	12
pubs.publication-status	Published online
pubs.volume	9
utslib.citation.issue	12

Abstract:

Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.

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http://hdl.handle.net/10453/155552