"Turn-on" Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells.

Ma, K; Zhang, F; Sayyadi, N; Chen, W; Anwer, AG; Care, A; Xu, B; Tian, W; Goldys, EM; Liu, G

"Turn-on" Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells.

Ma, K Zhang, F Sayyadi, N

Chen, W Anwer, AG Care, A

Xu, B Tian, W Goldys, EM Liu, G

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS Sens, 2018, 3, (2), pp. 320-326
Issue Date:: 2018-02-23

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Field	Value	Language
dc.contributor.author	Ma, K
dc.contributor.author	Zhang, F
dc.contributor.author	Sayyadi, N https://orcid.org/0000-0002-0044-2170
dc.contributor.author	Chen, W
dc.contributor.author	Anwer, AG
dc.contributor.author	Care, A https://orcid.org/0000-0002-0035-7961
dc.contributor.author	Xu, B
dc.contributor.author	Tian, W
dc.contributor.author	Goldys, EM
dc.contributor.author	Liu, G
dc.date.accessioned	2022-03-25T04:50:00Z
dc.date.available	2022-03-25T04:50:00Z
dc.date.issued	2018-02-23
dc.identifier.citation	ACS Sens, 2018, 3, (2), pp. 320-326
dc.identifier.issn	2379-3694
dc.identifier.issn	2379-3694
dc.identifier.uri	http://hdl.handle.net/10453/155566
dc.description.abstract	We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL-1, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL-1, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation.ispartof	ACS Sens
dc.relation.isbasedon	10.1021/acssensors.7b00720
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0903 Biomedical Engineering, 1007 Nanotechnology
dc.subject.mesh	Animals
dc.subject.mesh	Aptamers, Nucleotide
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Cell Line
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Interferon-gamma
dc.subject.mesh	Mice
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Cell Line
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Aptamers, Nucleotide
dc.subject.mesh	Interferon-gamma
dc.title	"Turn-on" Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells.
dc.type	Journal Article
utslib.citation.volume	3
utslib.location.activity	United States
utslib.for	0301 Analytical Chemistry
utslib.for	0903 Biomedical Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2022-03-25T04:49:58Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	2

Abstract:

We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL-1, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL-1, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.

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http://hdl.handle.net/10453/155566