Productivity and community response along an ammonia gradient in cultured wild marine microalgae, using wastewater-derived nutrients for cost-effective feedstock production

Sutherland, DL; Ralph, PJ

Productivity and community response along an ammonia gradient in cultured wild marine microalgae, using wastewater-derived nutrients for cost-effective feedstock production

Sutherland, DL Ralph, PJ

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Journal of Applied Phycology, 2021, 33, (5), pp. 2933-2945
Issue Date:: 2021-10-01

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Field	Value	Language
dc.contributor.author	Sutherland, DL
dc.contributor.author	Ralph, PJ
dc.date.accessioned	2022-03-27T23:40:42Z
dc.date.available	2022-03-27T23:40:42Z
dc.date.issued	2021-10-01
dc.identifier.citation	Journal of Applied Phycology, 2021, 33, (5), pp. 2933-2945
dc.identifier.issn	0921-8971
dc.identifier.issn	1573-5176
dc.identifier.uri	http://hdl.handle.net/10453/155622
dc.description.abstract	Wild marine microalgal consortia may overcome a number of challenges of growing domesticated strains in outdoor open pond systems, such as poor tolerance of fluctuating environment and pond crashes. We investigated the performance of wild marine microalgal communities grown under four ammoniacal nitrogen loads: (i) 6.25 g m−3 (0.5 ×), (ii) 12.5 g m−3 (1 ×), (iii) 25 g m−3 (2 ×) and (iv) 50 g m−3 (4 ×), with respect to biomass yield, macromolecular composition and community membership. Volatile suspended solids (organic matter) increased with increasing ammonia load, with 4 × (210 ± 15 g m−3) significantly higher (p < 0.01) than 2 × (167 ± 24 g m−3), 1 × (127 ± 8 g m−3) and 0.5 × (97 ± 13 g m−3), but the response was not linear. Supplemental dissolved inorganic phosphorus did not increase overall biomass production. Percentage protein per unit biomass significantly increased with increased ammonia, with 2 × and 4 × significantly higher (p < 0.05) than 0.5 × and 1 × , but this came at the expense of carbohydrates. Percentage carbohydrate was significantly lower (p < 0.01) in the 2 × and 4 × treatments. A total of 13 species, including eight dominant species, were recorded across all treatments. As ammonia increased, the community shifted from a diatom (Amphora ovalis, Coscinodiscus radiatus, Minidiscus sp.)-dominated community (by biovolume) to a green alga–dominated community, while Nannochloropsis sp. was numerically dominant across all treatments. These results indicate the capability of marine microalgae to utilise waste centrate as a rich nutrient source. Further investigations under outdoor conditions, where multiple environmental stressors may intensify differential responses amongst individual species, are needed to better understand community response.
dc.language	English
dc.publisher	SPRINGER
dc.relation.ispartof	Journal of Applied Phycology
dc.relation.isbasedon	10.1007/s10811-021-02520-1
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0607 Plant Biology, 0704 Fisheries Sciences, 1002 Environmental Biotechnology
dc.subject.classification	Marine Biology & Hydrobiology
dc.title	Productivity and community response along an ammonia gradient in cultured wild marine microalgae, using wastewater-derived nutrients for cost-effective feedstock production
dc.type	Journal Article
utslib.citation.volume	33
utslib.for	0607 Plant Biology
utslib.for	0704 Fisheries Sciences
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access	*
dc.date.updated	2022-03-27T23:40:37Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	33
utslib.citation.issue	5

Abstract:

Wild marine microalgal consortia may overcome a number of challenges of growing domesticated strains in outdoor open pond systems, such as poor tolerance of fluctuating environment and pond crashes. We investigated the performance of wild marine microalgal communities grown under four ammoniacal nitrogen loads: (i) 6.25 g m−3 (0.5 ×), (ii) 12.5 g m−3 (1 ×), (iii) 25 g m−3 (2 ×) and (iv) 50 g m−3 (4 ×), with respect to biomass yield, macromolecular composition and community membership. Volatile suspended solids (organic matter) increased with increasing ammonia load, with 4 × (210 ± 15 g m−3) significantly higher (p < 0.01) than 2 × (167 ± 24 g m−3), 1 × (127 ± 8 g m−3) and 0.5 × (97 ± 13 g m−3), but the response was not linear. Supplemental dissolved inorganic phosphorus did not increase overall biomass production. Percentage protein per unit biomass significantly increased with increased ammonia, with 2 × and 4 × significantly higher (p < 0.05) than 0.5 × and 1 × , but this came at the expense of carbohydrates. Percentage carbohydrate was significantly lower (p < 0.01) in the 2 × and 4 × treatments. A total of 13 species, including eight dominant species, were recorded across all treatments. As ammonia increased, the community shifted from a diatom (Amphora ovalis, Coscinodiscus radiatus, Minidiscus sp.)-dominated community (by biovolume) to a green alga–dominated community, while Nannochloropsis sp. was numerically dominant across all treatments. These results indicate the capability of marine microalgae to utilise waste centrate as a rich nutrient source. Further investigations under outdoor conditions, where multiple environmental stressors may intensify differential responses amongst individual species, are needed to better understand community response.

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http://hdl.handle.net/10453/155622