Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration

Hansbro, P; Ashcroft, SP; Fletcher, G; Philp, AM; Jenkinson, C; Handelsman, DJ; Das, S; Atherton, PJ; Philp, A

Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration

Hansbro, P

Ashcroft, SP Fletcher, G Philp, AM Jenkinson, C Handelsman, DJ Das, S Atherton, PJ Philp, A

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Publisher:: BioScientifica
Publication Type:: Journal Article
Citation:: Journal of Endocrinology, 2021, 249, (2), pp. 113-124
Issue Date:: 2021-03-10

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Field	Value	Language
dc.contributor.author	Hansbro, P https://orcid.org/0000-0002-4741-3035
dc.contributor.author	Ashcroft, SP
dc.contributor.author	Fletcher, G
dc.contributor.author	Philp, AM
dc.contributor.author	Jenkinson, C
dc.contributor.author	Handelsman, DJ
dc.contributor.author	Das, S
dc.contributor.author	Atherton, PJ
dc.contributor.author	Philp, A
dc.date.accessioned	2022-03-29T03:20:51Z
dc.date.available	2021-03-10
dc.date.available	2022-03-29T03:20:51Z
dc.date.issued	2021-03-10
dc.identifier.citation	Journal of Endocrinology, 2021, 249, (2), pp. 113-124
dc.identifier.issn	0022-0795
dc.identifier.issn	1479-6805
dc.identifier.uri	http://hdl.handle.net/10453/155661
dc.description.abstract	Vitamin D deficiency is associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D-related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to in vitro models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function in vivo have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function in vivo, we utilised an established model of diet-induced vitamin D deficiency in C57BL/6J mice. Mice were either fed a control diet (2200 IU/kg i.e. vitamin D replete) or a vitamin D-deplete (0 IU/kg) diet for periods of 1, 2 and 3 months. Gastrocnemius muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3 months of diet-induced vitamin D deficiency, respiration supported via complex I + II (CI + IIP) and the electron transport chain (ETC) were 35 and 37% lower when compared to vitamin D-replete mice (P < 0.05). Despite functional alterations, citrate synthase activity, AMPK phosphorylation, mitofilin, OPA1 and ETC subunit protein content remained unchanged in response to dietary intervention (P > 0.05). In conclusion, we report that 3 months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial respiration in C57BL/6J mice. Our data, when combined with previous in vitro observations, suggest that vitamin D-mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.
dc.format	Print
dc.language	eng
dc.publisher	BioScientifica
dc.relation.ispartof	Journal of Endocrinology
dc.relation.isbasedon	10.1530/JOE-20-0233
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0702 Animal Production, 0707 Veterinary Sciences, 1103 Clinical Sciences
dc.subject.classification	Endocrinology & Metabolism
dc.subject.mesh	Animals
dc.subject.mesh	Body Composition
dc.subject.mesh	Calcium
dc.subject.mesh	Diet
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Male
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mitochondria, Muscle
dc.subject.mesh	Mitochondrial Proteins
dc.subject.mesh	Muscle, Skeletal
dc.subject.mesh	Oxygen Consumption
dc.subject.mesh	Vitamin D
dc.subject.mesh	Vitamin D Deficiency
dc.subject.mesh	Muscle, Skeletal
dc.subject.mesh	Mitochondria, Muscle
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Vitamin D Deficiency
dc.subject.mesh	Calcium
dc.subject.mesh	Vitamin D
dc.subject.mesh	Mitochondrial Proteins
dc.subject.mesh	Diet
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Body Composition
dc.subject.mesh	Oxygen Consumption
dc.subject.mesh	Male
dc.title	Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration
dc.type	Journal Article
utslib.citation.volume	249
utslib.location.activity	England
utslib.for	0702 Animal Production
utslib.for	0707 Veterinary Sciences
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-29T03:20:49Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	249
utslib.citation.issue	2

Abstract:

Vitamin D deficiency is associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D-related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to in vitro models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function in vivo have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function in vivo, we utilised an established model of diet-induced vitamin D deficiency in C57BL/6J mice. Mice were either fed a control diet (2200 IU/kg i.e. vitamin D replete) or a vitamin D-deplete (0 IU/kg) diet for periods of 1, 2 and 3 months. Gastrocnemius muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3 months of diet-induced vitamin D deficiency, respiration supported via complex I + II (CI + IIP) and the electron transport chain (ETC) were 35 and 37% lower when compared to vitamin D-replete mice (P < 0.05). Despite functional alterations, citrate synthase activity, AMPK phosphorylation, mitofilin, OPA1 and ETC subunit protein content remained unchanged in response to dietary intervention (P > 0.05). In conclusion, we report that 3 months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial respiration in C57BL/6J mice. Our data, when combined with previous in vitro observations, suggest that vitamin D-mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.

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http://hdl.handle.net/10453/155661