The effect of parathyroid hormone on the uptake and retention of 25-hydroxyvitamin D in skeletal muscle cells.

Abboud, M; Rybchyn, MS; Liu, J; Ning, Y; Gordon-Thomson, C; Brennan-Speranza, TC; Cole, L; Greenfield, H; Fraser, DR; Mason, RS

The effect of parathyroid hormone on the uptake and retention of 25-hydroxyvitamin D in skeletal muscle cells.

Abboud, M Rybchyn, MS Liu, J Ning, Y Gordon-Thomson, C Brennan-Speranza, TC Cole, L

Greenfield, H Fraser, DR Mason, RS

Permalink

Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: J Steroid Biochem Mol Biol, 2017, 173, pp. 173-179
Issue Date:: 2017-10

Closed Access

	Filename	Description	Size
	1-s2.0-S0960076017300018-main.pdf		1.1 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Abboud, M
dc.contributor.author	Rybchyn, MS
dc.contributor.author	Liu, J
dc.contributor.author	Ning, Y
dc.contributor.author	Gordon-Thomson, C
dc.contributor.author	Brennan-Speranza, TC
dc.contributor.author	Cole, L https://orcid.org/0000-0001-9206-9272
dc.contributor.author	Greenfield, H
dc.contributor.author	Fraser, DR
dc.contributor.author	Mason, RS
dc.date.accessioned	2022-04-01T00:40:02Z
dc.date.available	2017-01-03
dc.date.available	2022-04-01T00:40:02Z
dc.date.issued	2017-10
dc.identifier.citation	J Steroid Biochem Mol Biol, 2017, 173, pp. 173-179
dc.identifier.issn	0960-0760
dc.identifier.issn	1879-1220
dc.identifier.uri	http://hdl.handle.net/10453/155840
dc.description.abstract	Data from our studies, and those of others, support the proposal that there is a role for skeletal muscle in the maintenance of vitamin D status. We demonstrated that skeletal muscle is able to internalise extracellular vitamin D binding protein, which then binds to actin in the cytoplasm, to provide high affinity binding sites which accumulate 25-hydroxyvitamin D3 (25(OH)D3) [1]. This study investigated the concentration- and time-dependent effects of parathyroid hormone (PTH) on the capacity of muscle cells to take up and release 3H-25(OH)D3. Uptake and retention studies for 3H-25(OH)D3 were carried out with C2C12 cells differentiated into myotubes and with primary mouse muscle fibers as described [1]. The presence of PTH receptors on mouse muscle fibers was demonstrated by immunohistochemistry and PTH receptors were detected in differentiated myotubes, but not myoblasts, and on muscle fibers by Western blot. Addition of low concentrations of vitamin D binding protein to the incubation media did not alter uptake of 25(OH)D3. Pre-incubation of C2 myotubes or primary mouse muscle fibers with PTH (0.1 to 100 pM) for 3h resulted in a concentration-dependent decrease in 25(OH)D3 uptake after 4 or 16h. These effects were significant at 0.1 or 1pM PTH (p<0.001) and plateaued at 10pM, with 25(OH)D3 uptake reduced by over 60% (p<0.001) in both cell types. In C2 myotubes, retention of 25(OH)D3 was decreased after addition of PTH (0.1 to 100pM) in a concentration-dependent manner by up to 80% (p<0.001) compared to non-PTH treated-C2 myotubes. These data show that muscle uptake and retention of 25(OH)D3 are modulated by PTH, a physiological regulator of mineral homeostasis, but the cell culture model may not be a comprehensive reflection of vitamin D homeostatic mechanisms in whole animals.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	J Steroid Biochem Mol Biol
dc.relation.isbasedon	10.1016/j.jsbmb.2017.01.001
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology
dc.subject.classification	Endocrinology & Metabolism
dc.subject.mesh	Animals
dc.subject.mesh	Cell Line
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Humans
dc.subject.mesh	Mice, Inbred BALB C
dc.subject.mesh	Muscle Fibers, Skeletal
dc.subject.mesh	Muscle, Skeletal
dc.subject.mesh	Parathyroid Hormone
dc.subject.mesh	Vitamin D
dc.subject.mesh	Muscle, Skeletal
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Cell Line
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred BALB C
dc.subject.mesh	Humans
dc.subject.mesh	Parathyroid Hormone
dc.subject.mesh	Vitamin D
dc.subject.mesh	Muscle Fibers, Skeletal
dc.title	The effect of parathyroid hormone on the uptake and retention of 25-hydroxyvitamin D in skeletal muscle cells.
dc.type	Journal Article
utslib.citation.volume	173
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access	*
dc.date.updated	2022-04-01T00:40:00Z
pubs.publication-status	Published
pubs.volume	173

Abstract:

Data from our studies, and those of others, support the proposal that there is a role for skeletal muscle in the maintenance of vitamin D status. We demonstrated that skeletal muscle is able to internalise extracellular vitamin D binding protein, which then binds to actin in the cytoplasm, to provide high affinity binding sites which accumulate 25-hydroxyvitamin D3 (25(OH)D3) [1]. This study investigated the concentration- and time-dependent effects of parathyroid hormone (PTH) on the capacity of muscle cells to take up and release 3H-25(OH)D3. Uptake and retention studies for 3H-25(OH)D3 were carried out with C2C12 cells differentiated into myotubes and with primary mouse muscle fibers as described [1]. The presence of PTH receptors on mouse muscle fibers was demonstrated by immunohistochemistry and PTH receptors were detected in differentiated myotubes, but not myoblasts, and on muscle fibers by Western blot. Addition of low concentrations of vitamin D binding protein to the incubation media did not alter uptake of 25(OH)D3. Pre-incubation of C2 myotubes or primary mouse muscle fibers with PTH (0.1 to 100 pM) for 3h resulted in a concentration-dependent decrease in 25(OH)D3 uptake after 4 or 16h. These effects were significant at 0.1 or 1pM PTH (p<0.001) and plateaued at 10pM, with 25(OH)D3 uptake reduced by over 60% (p<0.001) in both cell types. In C2 myotubes, retention of 25(OH)D3 was decreased after addition of PTH (0.1 to 100pM) in a concentration-dependent manner by up to 80% (p<0.001) compared to non-PTH treated-C2 myotubes. These data show that muscle uptake and retention of 25(OH)D3 are modulated by PTH, a physiological regulator of mineral homeostasis, but the cell culture model may not be a comprehensive reflection of vitamin D homeostatic mechanisms in whole animals.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/155840