Quorum Regulated Resistance of Vibrio cholerae against Environmental Bacteriophages.

Hoque, MM; Naser, IB; Bari, SMN; Zhu, J; Mekalanos, JJ; Faruque, SM

Quorum Regulated Resistance of Vibrio cholerae against Environmental Bacteriophages.

Hoque, MM Naser, IB Bari, SMN Zhu, J Mekalanos, JJ Faruque, SM

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Sci Rep, 2016, 6, (1), pp. 37956
Issue Date:: 2016-11-28

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Field	Value	Language
dc.contributor.author	Hoque, MM
dc.contributor.author	Naser, IB
dc.contributor.author	Bari, SMN
dc.contributor.author	Zhu, J
dc.contributor.author	Mekalanos, JJ
dc.contributor.author	Faruque, SM
dc.date.accessioned	2022-04-01T03:09:50Z
dc.date.available	2016-11-01
dc.date.available	2022-04-01T03:09:50Z
dc.date.issued	2016-11-28
dc.identifier.citation	Sci Rep, 2016, 6, (1), pp. 37956
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/155858
dc.description.abstract	Predation by bacteriophages can significantly influence the population structure of bacterial communities. Vibrio cholerae the causative agent of cholera epidemics interacts with numerous phages in the aquatic ecosystem, and in the intestine of cholera patients. Seasonal epidemics of cholera reportedly collapse due to predation of the pathogen by phages. However, it is not clear how sufficient number of the bacteria survive to seed the environment in the subsequent epidemic season. We found that bacterial cell density-dependent gene expression termed "quorum sensing" which is regulated by signal molecules called autoinducers (AIs) can protect V. cholerae against predatory phages. V. cholerae mutant strains carrying inactivated AI synthase genes were significantly more susceptible to multiple phages compared to the parent bacteria. Likewise when mixed cultures of phage and bacteria were supplemented with exogenous autoinducers CAI-1 or AI-2 produced by recombinant strains carrying cloned AI synthase genes, increased survival of V. cholerae and a decrease in phage titer was observed. Mutational analyses suggested that the observed effects of autoinducers are mediated in part through the quorum sensing-dependent production of haemaglutinin protease, and partly through downregulation of phage receptors. These results have implication in developing strategies for phage mediated control of cholera.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/srep37956
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Agglutination
dc.subject.mesh	Animals
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Bacteriophages
dc.subject.mesh	Down-Regulation
dc.subject.mesh	Gene Expression Regulation, Bacterial
dc.subject.mesh	Homoserine
dc.subject.mesh	Immune Sera
dc.subject.mesh	Ketones
dc.subject.mesh	Lactones
dc.subject.mesh	Mutation
dc.subject.mesh	O Antigens
dc.subject.mesh	Peptide Hydrolases
dc.subject.mesh	Quorum Sensing
dc.subject.mesh	Rabbits
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Animals
dc.subject.mesh	Rabbits
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Bacteriophages
dc.subject.mesh	Ketones
dc.subject.mesh	Lactones
dc.subject.mesh	Peptide Hydrolases
dc.subject.mesh	O Antigens
dc.subject.mesh	Homoserine
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Immune Sera
dc.subject.mesh	Agglutination
dc.subject.mesh	Down-Regulation
dc.subject.mesh	Gene Expression Regulation, Bacterial
dc.subject.mesh	Mutation
dc.subject.mesh	Quorum Sensing
dc.title	Quorum Regulated Resistance of Vibrio cholerae against Environmental Bacteriophages.
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-01T03:09:46Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	6
utslib.citation.issue	1

Abstract:

Predation by bacteriophages can significantly influence the population structure of bacterial communities. Vibrio cholerae the causative agent of cholera epidemics interacts with numerous phages in the aquatic ecosystem, and in the intestine of cholera patients. Seasonal epidemics of cholera reportedly collapse due to predation of the pathogen by phages. However, it is not clear how sufficient number of the bacteria survive to seed the environment in the subsequent epidemic season. We found that bacterial cell density-dependent gene expression termed "quorum sensing" which is regulated by signal molecules called autoinducers (AIs) can protect V. cholerae against predatory phages. V. cholerae mutant strains carrying inactivated AI synthase genes were significantly more susceptible to multiple phages compared to the parent bacteria. Likewise when mixed cultures of phage and bacteria were supplemented with exogenous autoinducers CAI-1 or AI-2 produced by recombinant strains carrying cloned AI synthase genes, increased survival of V. cholerae and a decrease in phage titer was observed. Mutational analyses suggested that the observed effects of autoinducers are mediated in part through the quorum sensing-dependent production of haemaglutinin protease, and partly through downregulation of phage receptors. These results have implication in developing strategies for phage mediated control of cholera.

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http://hdl.handle.net/10453/155858