Prophylactic, Therapeutic And Immune Enhancement Effect Of Liposome-Encapsulated Polyiclc On Highly Pathogenic H5N1 Influenza Infection

John Wiley & Sons Ltd
Publication Type:
Journal Article
Journal Of Gene Medicine, 2011, 13 (1), pp. 60 - 72
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Background In view of the magnitude and severity of outbreaks of the highly pathogenic H5N1 influenza virus (H5N1-HPIV) and the threat to public health, there is an urgent need to develop broad-spectrum prophylactic and therapeutic agents against infection by H5N1-HPIV and other subtypes. Methods and Results In the present study, we explored the use of LEPolyICLC, a liposome encapsulated double-stranded RNA, as a possible prophylactic, therapeutic and immune enhancement agent. In a mouse infection model, we showed that the administration of LE-PolyICLC intranasally before or shortly after infection could inhibit virus replication, leading to a significant reduction in pulmonary viral titres and a higher survival rate of infected mice. When used as a molecular adjuvant, LEPolyICLC significantly enhanced both the humoral and cellular responses elicited by inactivated H5N1 vaccine and augmented the protective efficacy provided by vaccination.Most importantly, the data also demonstrate that LEPolyICLC could effectively attenuate the development of pulmonary fibrosis during the restoration period at day 14 after H5N1 infection.
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