Ferric reducing antioxidant potential (FRAP) of antioxidants using reaction flow chromatography.

Jones, A; Pravadali-Cekic, S; Dennis, GR; Bashir, R; Mahon, PJ; Shalliker, RA

Ferric reducing antioxidant potential (FRAP) of antioxidants using reaction flow chromatography.

Jones, A Pravadali-Cekic, S Dennis, GR Bashir, R Mahon, PJ Shalliker, RA

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Analytica Chimica Acta, 2017, 967, pp. 93-101
Issue Date:: 2017-05-15

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Field	Value	Language
dc.contributor.author	Jones, A
dc.contributor.author	Pravadali-Cekic, S
dc.contributor.author	Dennis, GR
dc.contributor.author	Bashir, R
dc.contributor.author	Mahon, PJ
dc.contributor.author	Shalliker, RA
dc.date.accessioned	2022-07-08T04:25:56Z
dc.date.available	2017-02-13
dc.date.available	2022-07-08T04:25:56Z
dc.date.issued	2017-05-15
dc.identifier.citation	Analytica Chimica Acta, 2017, 967, pp. 93-101
dc.identifier.issn	0003-2670
dc.identifier.issn	1873-4324
dc.identifier.uri	http://hdl.handle.net/10453/158756
dc.description.abstract	High performance liquid chromatography coupled with post column derivatisation (HPLC-PCD) may be used to profile the antioxidant content of a sample. There are, however, drawbacks in the use of HPLC-PCD setups; namely the high volume reaction coils that are typically used lowering the observed separation efficiency. Reaction flow chromatography has the ability to overcome these inefficiencies by using a more efficient mixing technique inside the outlet fitting itself, post column reaction loops can be removed with resulting improvement in signal to noise response, plus the separation efficiency is maintained. We assessed two methods of HPLC-PCD antioxidant analysis based on the ferric reducing antioxidant power (FRAP) assay in both conventional and reaction flow HPLC-PCD modes. It was found that the reaction flow technique demonstrated significant advantages over the conventional technique in terms of signal to noise, linear range, precision and observed separation efficiency.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Analytica Chimica Acta
dc.relation.isbasedon	10.1016/j.aca.2017.02.032
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Ferric reducing antioxidant potential (FRAP) of antioxidants using reaction flow chromatography.
dc.type	Journal Article
utslib.citation.volume	967
utslib.location.activity	Netherlands
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-07-08T04:25:55Z
pubs.publication-status	Published
pubs.volume	967

Abstract:

High performance liquid chromatography coupled with post column derivatisation (HPLC-PCD) may be used to profile the antioxidant content of a sample. There are, however, drawbacks in the use of HPLC-PCD setups; namely the high volume reaction coils that are typically used lowering the observed separation efficiency. Reaction flow chromatography has the ability to overcome these inefficiencies by using a more efficient mixing technique inside the outlet fitting itself, post column reaction loops can be removed with resulting improvement in signal to noise response, plus the separation efficiency is maintained. We assessed two methods of HPLC-PCD antioxidant analysis based on the ferric reducing antioxidant power (FRAP) assay in both conventional and reaction flow HPLC-PCD modes. It was found that the reaction flow technique demonstrated significant advantages over the conventional technique in terms of signal to noise, linear range, precision and observed separation efficiency.

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http://hdl.handle.net/10453/158756