Protozoal food vacuoles enhance transformation in Vibrio cholerae through SOS-regulated DNA integration.

Rahman, MH; Mahbub, KR; Espinoza-Vergara, G; Ritchie, A; Hoque, MM; Noorian, P; Cole, L; McDougald, D; Labbate, M

Protozoal food vacuoles enhance transformation in Vibrio cholerae through SOS-regulated DNA integration.

Rahman, MH Mahbub, KR Espinoza-Vergara, G Ritchie, A Hoque, MM Noorian, P

Cole, L McDougald, D Labbate, M

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Publisher:: SPRINGERNATURE
Publication Type:: Journal Article
Citation:: ISME J, 2022
Issue Date:: 2022-05-16

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Field	Value	Language
dc.contributor.author	Rahman, MH
dc.contributor.author	Mahbub, KR
dc.contributor.author	Espinoza-Vergara, G
dc.contributor.author	Ritchie, A
dc.contributor.author	Hoque, MM
dc.contributor.author	Noorian, P https://orcid.org/0000-0002-4215-1080
dc.contributor.author	Cole, L
dc.contributor.author	McDougald, D
dc.contributor.author	Labbate, M https://orcid.org/0000-0003-1428-3382
dc.date.accessioned	2022-07-26T23:29:12Z
dc.date.available	2022-04-29
dc.date.available	2022-07-26T23:29:12Z
dc.date.issued	2022-05-16
dc.identifier.citation	ISME J, 2022
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/159190
dc.description.abstract	Vibrio cholerae, the bacterial pathogen responsible for the diarrheal disease cholera, resides in the aquatic environment between outbreaks. For bacteria, genetic variation by lateral gene transfer (LGT) is important for survival and adaptation. In the aquatic environment, V. cholerae is predominantly found in biofilms associated with chitinous organisms or with chitin "rain". Chitin induces competency in V. cholerae, which can lead to LGT. In the environment, V. cholerae is also subjected to predation pressure by protist. Here we investigated whether protozoal predation affected LGT using the integron as a model. Integrons facilitate the integration of mobile DNA (gene cassettes) into the bacterial chromosome. We report that protozoal predation enhances transformation of a gene cassette by as much as 405-fold. We show that oxidative radicals produced in the protozoal phagosome induces the universal SOS response, which in turn upregulates the integron-integrase, the recombinase that facilitates cassette integration. Additionally, we show that during predation, V. cholerae requires the type VI secretion system to acquire the gene cassette from Escherichia coli. These results show that protozoal predation enhances LGT thus producing genetic variants that may have increased capacity to survive grazing. Additionally, the conditions in the food vacuole may make it a "hot spot" for LGT by accumulating diverse bacteria and inducing the SOS response helping drive genetic diversification and evolution.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SPRINGERNATURE
dc.relation.ispartof	ISME J
dc.relation.isbasedon	10.1038/s41396-022-01249-0
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.title	Protozoal food vacuoles enhance transformation in Vibrio cholerae through SOS-regulated DNA integration.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-07-26T23:29:11Z
pubs.publication-status	Published online

Abstract:

Vibrio cholerae, the bacterial pathogen responsible for the diarrheal disease cholera, resides in the aquatic environment between outbreaks. For bacteria, genetic variation by lateral gene transfer (LGT) is important for survival and adaptation. In the aquatic environment, V. cholerae is predominantly found in biofilms associated with chitinous organisms or with chitin "rain". Chitin induces competency in V. cholerae, which can lead to LGT. In the environment, V. cholerae is also subjected to predation pressure by protist. Here we investigated whether protozoal predation affected LGT using the integron as a model. Integrons facilitate the integration of mobile DNA (gene cassettes) into the bacterial chromosome. We report that protozoal predation enhances transformation of a gene cassette by as much as 405-fold. We show that oxidative radicals produced in the protozoal phagosome induces the universal SOS response, which in turn upregulates the integron-integrase, the recombinase that facilitates cassette integration. Additionally, we show that during predation, V. cholerae requires the type VI secretion system to acquire the gene cassette from Escherichia coli. These results show that protozoal predation enhances LGT thus producing genetic variants that may have increased capacity to survive grazing. Additionally, the conditions in the food vacuole may make it a "hot spot" for LGT by accumulating diverse bacteria and inducing the SOS response helping drive genetic diversification and evolution.

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http://hdl.handle.net/10453/159190