A mobility shift assay for DNA detection using nanochannel gradient electrophoresis.

Startsev, MA; Ostrowski, M; Goldys, EM; Inglis, DW

A mobility shift assay for DNA detection using nanochannel gradient electrophoresis.

Startsev, MA Ostrowski, M

Goldys, EM Inglis, DW

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Publisher:: Wiley-VCH Verlag
Publication Type:: Journal Article
Citation:: Electrophoresis, 2017, 38, (2), pp. 335-341
Issue Date:: 2017-01

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Field	Value	Language
dc.contributor.author	Startsev, MA
dc.contributor.author	Ostrowski, M https://orcid.org/0000-0002-4357-3023
dc.contributor.author	Goldys, EM
dc.contributor.author	Inglis, DW
dc.date.accessioned	2022-08-01T03:29:47Z
dc.date.available	2016-08-03
dc.date.available	2022-08-01T03:29:47Z
dc.date.issued	2017-01
dc.identifier.citation	Electrophoresis, 2017, 38, (2), pp. 335-341
dc.identifier.issn	0173-0835
dc.identifier.issn	1522-2683
dc.identifier.uri	http://hdl.handle.net/10453/159426
dc.description.abstract	Conventional detection of pathogenic or other biological contamination relies on amplification of DNA using sequence-specific primers. Recent work in nanofluidics has shown very high concentration enhancement of biomolecules with some degree of simultaneous separation. This work demonstrates the combination of these two approaches by selectively concentrating a mobility-shifted hybridization product, potentially enabling rapid detection of rare DNA fragments such as highly specific 16S ribosomal DNA. We have performed conductivity gradient electrofocusing within nanofluidic channels and have shown concentration of hybridized peptide nucleic acids and DNA oligomers. We also show selectivity to single base-pair mismatch on 18-mer oligos. This approach may enable sensitive optical detection of small amounts of DNA.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley-VCH Verlag
dc.relation.ispartof	Electrophoresis
dc.relation.isbasedon	10.1002/elps.201600358
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology, 0904 Chemical Engineering
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	DNA
dc.subject.mesh	Electrophoretic Mobility Shift Assay
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Nanotechnology
dc.subject.mesh	Peptide Nucleic Acids
dc.subject.mesh	DNA
dc.subject.mesh	Electrophoretic Mobility Shift Assay
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Nanotechnology
dc.subject.mesh	Peptide Nucleic Acids
dc.subject.mesh	DNA
dc.subject.mesh	Peptide Nucleic Acids
dc.subject.mesh	Electrophoretic Mobility Shift Assay
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Nanotechnology
dc.title	A mobility shift assay for DNA detection using nanochannel gradient electrophoresis.
dc.type	Journal Article
utslib.citation.volume	38
utslib.location.activity	Germany
utslib.for	0301 Analytical Chemistry
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0904 Chemical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-01T03:29:45Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	38
utslib.citation.issue	2

Abstract:

Conventional detection of pathogenic or other biological contamination relies on amplification of DNA using sequence-specific primers. Recent work in nanofluidics has shown very high concentration enhancement of biomolecules with some degree of simultaneous separation. This work demonstrates the combination of these two approaches by selectively concentrating a mobility-shifted hybridization product, potentially enabling rapid detection of rare DNA fragments such as highly specific 16S ribosomal DNA. We have performed conductivity gradient electrofocusing within nanofluidic channels and have shown concentration of hybridized peptide nucleic acids and DNA oligomers. We also show selectivity to single base-pair mismatch on 18-mer oligos. This approach may enable sensitive optical detection of small amounts of DNA.

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http://hdl.handle.net/10453/159426