Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules.

Su, J; Raghuwanshi, VS; Raverty, W; Garvey, CJ; Holden, PJ; Gillon, M; Holt, SA; Tabor, R; Batchelor, W; Garnier, G

Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules.

Su, J Raghuwanshi, VS Raverty, W Garvey, CJ Holden, PJ Gillon, M Holt, SA Tabor, R Batchelor, W Garnier, G

Permalink

Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Sci Rep, 2016, 6, (1), pp. 36119
Issue Date:: 2016-10-31

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Download Published versionAdobe PDF (2.84 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Su, J
dc.contributor.author	Raghuwanshi, VS
dc.contributor.author	Raverty, W
dc.contributor.author	Garvey, CJ
dc.contributor.author	Holden, PJ
dc.contributor.author	Gillon, M
dc.contributor.author	Holt, SA
dc.contributor.author	Tabor, R
dc.contributor.author	Batchelor, W
dc.contributor.author	Garnier, G
dc.date.accessioned	2022-08-21T21:24:59Z
dc.date.available	2016-10-11
dc.date.available	2022-08-21T21:24:59Z
dc.date.issued	2016-10-31
dc.identifier.citation	Sci Rep, 2016, 6, (1), pp. 36119
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/160613
dc.description.abstract	Novel thin and smooth deuterated cellulose films were synthesised to visualize adsorbed bio-macromolecules using contrast variation neutron reflectivity (NR) measurements. Incorporation of varying degrees of deuteration into cellulose was achieved by growing Gluconacetobacter xylinus in deuterated glycerol as carbon source dissolved in growth media containing D2O. The derivative of deuterated cellulose was prepared by trimethylsilylation(TMS) in ionic liquid(1-butyl-3-methylimidazolium chloride). The TMS derivative was dissolved in toluene for thin film preparation by spin-coating. The resulting film was regenerated into deuterated cellulose by exposure to acidic vapour. A common enzyme, horseradish peroxidase (HRP), was adsorbed from solution onto the deuterated cellulose films and visualized by NR. The scattering length density contrast of the deuterated cellulose enabled accurate visualization and quantification of the adsorbed HRP, which would have been impossible to achieve with non-deuterated cellulose. The procedure described enables preparing deuterated cellulose films that allows differentiation of cellulose and non-deuterated bio-macromolecules using NR.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/srep36119
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Adsorption
dc.subject.mesh	Cellulose
dc.subject.mesh	Deuterium
dc.subject.mesh	Deuterium Oxide
dc.subject.mesh	Gluconacetobacter
dc.subject.mesh	Horseradish Peroxidase
dc.subject.mesh	Imidazoles
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Neutron Diffraction
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared
dc.subject.mesh	Trimethylsilyl Compounds
dc.subject.mesh	Gluconacetobacter
dc.subject.mesh	Deuterium
dc.subject.mesh	Deuterium Oxide
dc.subject.mesh	Trimethylsilyl Compounds
dc.subject.mesh	Imidazoles
dc.subject.mesh	Cellulose
dc.subject.mesh	Horseradish Peroxidase
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Neutron Diffraction
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared
dc.subject.mesh	Adsorption
dc.title	Smooth deuterated cellulose films for the visualisation of adsorbed bio-macromolecules.
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-08-21T21:24:57Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	6
utslib.citation.issue	1

Abstract:

Novel thin and smooth deuterated cellulose films were synthesised to visualize adsorbed bio-macromolecules using contrast variation neutron reflectivity (NR) measurements. Incorporation of varying degrees of deuteration into cellulose was achieved by growing Gluconacetobacter xylinus in deuterated glycerol as carbon source dissolved in growth media containing D2O. The derivative of deuterated cellulose was prepared by trimethylsilylation(TMS) in ionic liquid(1-butyl-3-methylimidazolium chloride). The TMS derivative was dissolved in toluene for thin film preparation by spin-coating. The resulting film was regenerated into deuterated cellulose by exposure to acidic vapour. A common enzyme, horseradish peroxidase (HRP), was adsorbed from solution onto the deuterated cellulose films and visualized by NR. The scattering length density contrast of the deuterated cellulose enabled accurate visualization and quantification of the adsorbed HRP, which would have been impossible to achieve with non-deuterated cellulose. The procedure described enables preparing deuterated cellulose films that allows differentiation of cellulose and non-deuterated bio-macromolecules using NR.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/160613