Efficiencies of recovery and extraction of trace DNA from non-porous surfaces

Wood, I; Park, S; Tooke, J; Smith, O; Morgan, RM; Meakin, GE

Efficiencies of recovery and extraction of trace DNA from non-porous surfaces

Wood, I Park, S Tooke, J Smith, O Morgan, RM Meakin, GE

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Forensic Science International: Genetics Supplement Series, 2017, 6, pp. e153-e155
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Wood, I
dc.contributor.author	Park, S
dc.contributor.author	Tooke, J
dc.contributor.author	Smith, O
dc.contributor.author	Morgan, RM
dc.contributor.author	Meakin, GE
dc.date.accessioned	2022-08-26T04:10:54Z
dc.date.available	2022-08-26T04:10:54Z
dc.date.issued	2017-12-01
dc.identifier.citation	Forensic Science International: Genetics Supplement Series, 2017, 6, pp. e153-e155
dc.identifier.issn	1875-1768
dc.identifier.issn	1875-175X
dc.identifier.uri	http://hdl.handle.net/10453/160896
dc.description.abstract	DNA recovery and extraction efficiencies are key considerations for trace DNA interpretation in casework, but prior studies have tended to focus on assessing these for body fluids rather than trace DNA. This study therefore examined the recovery and extraction of trace DNA using different collection methods from a range of non-porous surfaces relevant to crimes including homicides, terror attacks, and wildlife poaching. Direct extraction of DNA from solutions of a known concentration revealed absolute extraction efficiencies of ∼82%. When DNA was extracted from swabs seeded with the DNA solution, a similarly high efficiency of ∼85% was achieved from nylon-flocked swabs, with a lower efficiency of ∼55% from cotton swabs. However, when DNA was recovered from non-porous surfaces with swabs, ∼55% of DNA was still recovered from plastic knife handles, but lower efficiencies were achieved from the other substrates, particularly metal cable. Varied and poor recovery was observed using mini-tapes and requires further investigation. These results demonstrate that >50% recovery efficiency of trace DNA is achievable with both swab types, although recovery rates may be affected by surface type and/or practitioner experience.
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Forensic Science International: Genetics Supplement Series
dc.relation.isbasedon	10.1016/j.fsigss.2017.09.022
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Legal & Forensic Medicine
dc.title	Efficiencies of recovery and extraction of trace DNA from non-porous surfaces
dc.type	Journal Article
utslib.citation.volume	6
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-26T04:10:53Z
pubs.publication-status	Published
pubs.volume	6

Abstract:

DNA recovery and extraction efficiencies are key considerations for trace DNA interpretation in casework, but prior studies have tended to focus on assessing these for body fluids rather than trace DNA. This study therefore examined the recovery and extraction of trace DNA using different collection methods from a range of non-porous surfaces relevant to crimes including homicides, terror attacks, and wildlife poaching. Direct extraction of DNA from solutions of a known concentration revealed absolute extraction efficiencies of ∼82%. When DNA was extracted from swabs seeded with the DNA solution, a similarly high efficiency of ∼85% was achieved from nylon-flocked swabs, with a lower efficiency of ∼55% from cotton swabs. However, when DNA was recovered from non-porous surfaces with swabs, ∼55% of DNA was still recovered from plastic knife handles, but lower efficiencies were achieved from the other substrates, particularly metal cable. Varied and poor recovery was observed using mini-tapes and requires further investigation. These results demonstrate that >50% recovery efficiency of trace DNA is achievable with both swab types, although recovery rates may be affected by surface type and/or practitioner experience.

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http://hdl.handle.net/10453/160896