Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: A fast new approach to selective derivatization techniques

Pravadali-Cekic, S; Jones, A; Kazarian, AA; Paull, B; Soliven, A; Ritchie, H; Camenzuli, M; Leung, L; Dennis, GR; Shalliker, RA

Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: A fast new approach to selective derivatization techniques

Pravadali-Cekic, S Jones, A Kazarian, AA Paull, B Soliven, A Ritchie, H Camenzuli, M Leung, L Dennis, GR Shalliker, RA

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Publisher:: Taylor and Francis Group
Publication Type:: Journal Article
Citation:: Journal of Liquid Chromatography and Related Technologies, 2017, 40, (2), pp. 83-92
Issue Date:: 2017-01-20

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Field	Value	Language
dc.contributor.author	Pravadali-Cekic, S
dc.contributor.author	Jones, A
dc.contributor.author	Kazarian, AA
dc.contributor.author	Paull, B
dc.contributor.author	Soliven, A
dc.contributor.author	Ritchie, H
dc.contributor.author	Camenzuli, M
dc.contributor.author	Leung, L
dc.contributor.author	Dennis, GR
dc.contributor.author	Shalliker, RA
dc.date.accessioned	2022-09-01T02:00:04Z
dc.date.available	2022-09-01T02:00:04Z
dc.date.issued	2017-01-20
dc.identifier.citation	Journal of Liquid Chromatography and Related Technologies, 2017, 40, (2), pp. 83-92
dc.identifier.issn	1082-6076
dc.identifier.issn	1520-572X
dc.identifier.uri	http://hdl.handle.net/10453/161157
dc.description.abstract	Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.
dc.language	en
dc.publisher	Taylor and Francis Group
dc.relation.ispartof	Journal of Liquid Chromatography and Related Technologies
dc.relation.isbasedon	10.1080/10826076.2017.1285312
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: A fast new approach to selective derivatization techniques
dc.type	Journal Article
utslib.citation.volume	40
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-09-01T02:00:02Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	40
utslib.citation.issue	2

Abstract:

Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.

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http://hdl.handle.net/10453/161157