Hydroalcoholic extract of Argyreia speciosa roots ameliorates HCl-mediated acute lung injury in mice

Kaur, G; Jaswal, P; Banga, R; Dharwal, V; Kumar, A; Naura, A

Hydroalcoholic extract of Argyreia speciosa roots ameliorates HCl-mediated acute lung injury in mice

Kaur, G Jaswal, P Banga, R Dharwal, V

Kumar, A Naura, A

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Publisher:: WOLTERS KLUWER MEDKNOW PUBLICATIONS
Publication Type:: Journal Article
Citation:: Pharmacognosy Magazine, 2018, 14, (55), pp. S8-S13
Issue Date:: 2018-04-01

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Field	Value	Language
dc.contributor.author	Kaur, G
dc.contributor.author	Jaswal, P
dc.contributor.author	Banga, R
dc.contributor.author	Dharwal, V https://orcid.org/0000-0002-9645-3797
dc.contributor.author	Kumar, A
dc.contributor.author	Naura, A
dc.date.accessioned	2022-09-03T20:37:17Z
dc.date.available	2022-09-03T20:37:17Z
dc.date.issued	2018-04-01
dc.identifier.citation	Pharmacognosy Magazine, 2018, 14, (55), pp. S8-S13
dc.identifier.issn	0973-1296
dc.identifier.issn	0976-4062
dc.identifier.uri	http://hdl.handle.net/10453/161273
dc.description.abstract	Objective: Argyreia speciosa is a popular Indian medicinal plant. It has long been used in the traditional Ayurvedic Indian medicine for various diseases. The present work was designed to evaluate anti-inflammatory potential of hydroalcoholic extract of A. speciosa roots using mouse model of HCl-induced acute lung injury (ALI) by conducting a dose-response studies. Materials and Methods: The extract was given once orally at a dose of 50, 100, or 200 mg/kg b.wt. 90 min before HCl administration. Lung tissue of different group of mice was analyzed for the recruitment of inflammatory cells, redox status, and mRNA expression of pro-inflammatory genes to evaluate the anti-inflammatory effects of the extract. Results: Our results showed that the extract reduced HCl-induced lung inflammation in a dose-dependent manner. The neutrophil numbers in bronchoalveolar lavage fluid (BALF) were almost completely abrogated at a dose of 100 mg/kg b.wt. The severe reduction in neutrophils was accompanied with reduced pulmonary edema as the total protein content in BALF was found to be decreased substantially. In addition, the extract prevented the HCl-mediated oxidative stress in lungs as reflected by the normalization of levels of reactive oxygen species, malondialdehyde, redox status, and catalase activity. Extract seems to blunt the activity of redox-sensitive transcription factor nuclear factor-kappa B (NF-κB) as it suppressed the gene expression of ALI-linked pro-inflammatory cytokines, namely, interleukin-1 β and tumor necrosis factor-alpha. Conclusion: Overall, our data provide evidence that cocktail of natural phytochemicals present in hydroalcoholic extract of A. speciosa protects against HCl-induced ALI in mice potentially by curbing oxidative stress and consequent activation of NF-κB in the tissue.
dc.language	English
dc.publisher	WOLTERS KLUWER MEDKNOW PUBLICATIONS
dc.relation.ispartof	Pharmacognosy Magazine
dc.relation.isbasedon	10.4103/pm.pm_324_17
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Pharmacology & Pharmacy
dc.title	Hydroalcoholic extract of Argyreia speciosa roots ameliorates HCl-mediated acute lung injury in mice
dc.type	Journal Article
utslib.citation.volume	14
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2022-09-03T20:37:16Z
pubs.issue	55
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	55

Abstract:

Objective: Argyreia speciosa is a popular Indian medicinal plant. It has long been used in the traditional Ayurvedic Indian medicine for various diseases. The present work was designed to evaluate anti-inflammatory potential of hydroalcoholic extract of A. speciosa roots using mouse model of HCl-induced acute lung injury (ALI) by conducting a dose-response studies. Materials and Methods: The extract was given once orally at a dose of 50, 100, or 200 mg/kg b.wt. 90 min before HCl administration. Lung tissue of different group of mice was analyzed for the recruitment of inflammatory cells, redox status, and mRNA expression of pro-inflammatory genes to evaluate the anti-inflammatory effects of the extract. Results: Our results showed that the extract reduced HCl-induced lung inflammation in a dose-dependent manner. The neutrophil numbers in bronchoalveolar lavage fluid (BALF) were almost completely abrogated at a dose of 100 mg/kg b.wt. The severe reduction in neutrophils was accompanied with reduced pulmonary edema as the total protein content in BALF was found to be decreased substantially. In addition, the extract prevented the HCl-mediated oxidative stress in lungs as reflected by the normalization of levels of reactive oxygen species, malondialdehyde, redox status, and catalase activity. Extract seems to blunt the activity of redox-sensitive transcription factor nuclear factor-kappa B (NF-κB) as it suppressed the gene expression of ALI-linked pro-inflammatory cytokines, namely, interleukin-1 β and tumor necrosis factor-alpha. Conclusion: Overall, our data provide evidence that cocktail of natural phytochemicals present in hydroalcoholic extract of A. speciosa protects against HCl-induced ALI in mice potentially by curbing oxidative stress and consequent activation of NF-κB in the tissue.

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http://hdl.handle.net/10453/161273