Beneficial effects of Caesalpinia digyna extract against acid aspiration-Induced acute lung injury in mice

Chaudhary, M; Sharma, P; Mittal, M; Kaur, R; Dharwal, V; Kumar, A; Naura, AS

Beneficial effects of Caesalpinia digyna extract against acid aspiration-Induced acute lung injury in mice

Chaudhary, M Sharma, P Mittal, M Kaur, R Dharwal, V

Kumar, A Naura, AS

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Publisher:: Medknow Publications
Publication Type:: Journal Article
Citation:: Pharmacognosy Research, 2018, 10, (3), pp. 243-249
Issue Date:: 2018-07-01

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Field	Value	Language
dc.contributor.author	Chaudhary, M
dc.contributor.author	Sharma, P
dc.contributor.author	Mittal, M
dc.contributor.author	Kaur, R
dc.contributor.author	Dharwal, V https://orcid.org/0000-0002-9645-3797
dc.contributor.author	Kumar, A
dc.contributor.author	Naura, AS
dc.date.accessioned	2022-09-03T20:41:03Z
dc.date.available	2022-09-03T20:41:03Z
dc.date.issued	2018-07-01
dc.identifier.citation	Pharmacognosy Research, 2018, 10, (3), pp. 243-249
dc.identifier.issn	0974-8490
dc.identifier.issn	0974-8490
dc.identifier.uri	http://hdl.handle.net/10453/161274
dc.description.abstract	Objective: Caesalpinia digyna belongs to the genus Caesalpinia, which is known since ancient times for its medicinal properties. The present work was designed to evaluate the beneficial potential of hydroalcoholic extract of the roots of C. dignea against hydrochloric acid (HCl)-induced acute lung injury in mice. Materials and Methods: Ethanolic extract of C. dignea roots at a dose of 50, 100, or 200 mg/kg boy weight was given once orally 90 min before HCl administration. Mice were then analyzed for infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) and oxidative stress markers in the lung tissue. Further, the effects of the extract were compared with bergenin isolated from the extract. Results: Our results showed that an oral administration of the extract 90 min before HCl instillation reduced the infiltration of neutrophils in the lungs in a dose-dependent manner. Reduction in lung inflammation was associated with decline in pulmonary edema as the total protein content in the BALF was found to be decreased substantially. The drug also restored the redox balance in the lungs toward normal on HCl treatment as assessed by measuring the levels of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and catalase activity. Bergenin, isolated from the plant, was able to suppress the neutrophils but increased the macrophage number in BALF when administered before HCl instillation, suggesting immunoregulatory properties of the key constituent of the extract. Conclusion: Our data suggest that hydroalcoholic extract of Caesalpinia digyna roots constitute the phytochemicals that can protect against HCl-induced acute lung injury in mice..
dc.language	en
dc.publisher	Medknow Publications
dc.relation.ispartof	Pharmacognosy Research
dc.relation.isbasedon	10.4103/pr.pr-4-18
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0607 Plant Biology, 1104 Complementary and Alternative Medicine, 1115 Pharmacology and Pharmaceutical Sciences
dc.title	Beneficial effects of Caesalpinia digyna extract against acid aspiration-Induced acute lung injury in mice
dc.type	Journal Article
utslib.citation.volume	10
utslib.for	0607 Plant Biology
utslib.for	1104 Complementary and Alternative Medicine
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-09-03T20:41:01Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	3

Abstract:

Objective: Caesalpinia digyna belongs to the genus Caesalpinia, which is known since ancient times for its medicinal properties. The present work was designed to evaluate the beneficial potential of hydroalcoholic extract of the roots of C. dignea against hydrochloric acid (HCl)-induced acute lung injury in mice. Materials and Methods: Ethanolic extract of C. dignea roots at a dose of 50, 100, or 200 mg/kg boy weight was given once orally 90 min before HCl administration. Mice were then analyzed for infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) and oxidative stress markers in the lung tissue. Further, the effects of the extract were compared with bergenin isolated from the extract. Results: Our results showed that an oral administration of the extract 90 min before HCl instillation reduced the infiltration of neutrophils in the lungs in a dose-dependent manner. Reduction in lung inflammation was associated with decline in pulmonary edema as the total protein content in the BALF was found to be decreased substantially. The drug also restored the redox balance in the lungs toward normal on HCl treatment as assessed by measuring the levels of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and catalase activity. Bergenin, isolated from the plant, was able to suppress the neutrophils but increased the macrophage number in BALF when administered before HCl instillation, suggesting immunoregulatory properties of the key constituent of the extract. Conclusion: Our data suggest that hydroalcoholic extract of Caesalpinia digyna roots constitute the phytochemicals that can protect against HCl-induced acute lung injury in mice..

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http://hdl.handle.net/10453/161274