Analysis of mycobacterial infection-induced changes to host lipid metabolism in a zebrafish infection model reveals a conserved role for LDLR in infection susceptibility.

Johansen, MD; Hortle, E; Kasparian, JA; Romero, A; Novoa, B; Figueras, A; Britton, WJ; de Silva, K; Purdie, AC; Oehlers, SH

Analysis of mycobacterial infection-induced changes to host lipid metabolism in a zebrafish infection model reveals a conserved role for LDLR in infection susceptibility.

Johansen, MD Hortle, E

Kasparian, JA Romero, A Novoa, B Figueras, A Britton, WJ de Silva, K Purdie, AC Oehlers, SH

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Publisher:: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Fish Shellfish Immunol, 2018, 83, pp. 238-242
Issue Date:: 2018-12

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Field	Value	Language
dc.contributor.author	Johansen, MD
dc.contributor.author	Hortle, E https://orcid.org/0000-0001-9633-5638
dc.contributor.author	Kasparian, JA
dc.contributor.author	Romero, A
dc.contributor.author	Novoa, B
dc.contributor.author	Figueras, A
dc.contributor.author	Britton, WJ
dc.contributor.author	de Silva, K
dc.contributor.author	Purdie, AC
dc.contributor.author	Oehlers, SH
dc.date.accessioned	2022-09-05T22:52:30Z
dc.date.available	2018-09-12
dc.date.available	2022-09-05T22:52:30Z
dc.date.issued	2018-12
dc.identifier.citation	Fish Shellfish Immunol, 2018, 83, pp. 238-242
dc.identifier.issn	1050-4648
dc.identifier.issn	1095-9947
dc.identifier.uri	http://hdl.handle.net/10453/161392
dc.description.abstract	Changes to lipid metabolism are well-characterised consequences of human tuberculosis infection but their functional relevance are not clearly elucidated in these or other host-mycobacterial systems. The zebrafish-Mycobacterium marinum infection model is used extensively to model many aspects of human-M. tuberculosis pathogenesis but has not been widely used to study the role of infection-induced lipid metabolism. We find mammalian mycobacterial infection-induced alterations in host Low Density Lipoprotein metabolism are conserved in the zebrafish model of mycobacterial pathogenesis. Depletion of LDLR, a key lipid metabolism node, decreased M. marinum burden, and corrected infection-induced altered lipid metabolism resulting in decreased LDL and reduced the rate of macrophage transformation into foam cells. Our results demonstrate a conserved role for infection-induced alterations to host lipid metabolism, and specifically the LDL-LDLR axis, across host-mycobacterial species pairings.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
dc.relation.ispartof	Fish Shellfish Immunol
dc.relation.isbasedon	10.1016/j.fsi.2018.09.037
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0608 Zoology, 0704 Fisheries Sciences, 0707 Veterinary Sciences
dc.subject.classification	Fisheries
dc.subject.mesh	Animals
dc.subject.mesh	Cholesterol, LDL
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Embryo, Nonmammalian
dc.subject.mesh	Fish Diseases
dc.subject.mesh	Lipid Metabolism
dc.subject.mesh	Mycobacterium Infections, Nontuberculous
dc.subject.mesh	Receptors, LDL
dc.subject.mesh	Zebrafish
dc.subject.mesh	Zebrafish Proteins
dc.subject.mesh	Embryo, Nonmammalian
dc.subject.mesh	Animals
dc.subject.mesh	Zebrafish
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Fish Diseases
dc.subject.mesh	Zebrafish Proteins
dc.subject.mesh	Receptors, LDL
dc.subject.mesh	Lipid Metabolism
dc.subject.mesh	Cholesterol, LDL
dc.subject.mesh	Mycobacterium Infections, Nontuberculous
dc.title	Analysis of mycobacterial infection-induced changes to host lipid metabolism in a zebrafish infection model reveals a conserved role for LDLR in infection susceptibility.
dc.type	Journal Article
utslib.citation.volume	83
utslib.location.activity	England
utslib.for	0608 Zoology
utslib.for	0704 Fisheries Sciences
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-05T22:52:28Z
pubs.publication-status	Published
pubs.volume	83

Abstract:

Changes to lipid metabolism are well-characterised consequences of human tuberculosis infection but their functional relevance are not clearly elucidated in these or other host-mycobacterial systems. The zebrafish-Mycobacterium marinum infection model is used extensively to model many aspects of human-M. tuberculosis pathogenesis but has not been widely used to study the role of infection-induced lipid metabolism. We find mammalian mycobacterial infection-induced alterations in host Low Density Lipoprotein metabolism are conserved in the zebrafish model of mycobacterial pathogenesis. Depletion of LDLR, a key lipid metabolism node, decreased M. marinum burden, and corrected infection-induced altered lipid metabolism resulting in decreased LDL and reduced the rate of macrophage transformation into foam cells. Our results demonstrate a conserved role for infection-induced alterations to host lipid metabolism, and specifically the LDL-LDLR axis, across host-mycobacterial species pairings.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/161392