A tethered bilayer lipid membrane that mimics microbial membranes.

Andersson, J; Fuller, MA; Wood, K; Holt, SA; Köper, I

A tethered bilayer lipid membrane that mimics microbial membranes.

Andersson, J Fuller, MA Wood, K Holt, SA Köper, I

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Phys Chem Chem Phys, 2018, 20, (18), pp. 12958-12969
Issue Date:: 2018-05-09

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Field	Value	Language
dc.contributor.author	Andersson, J
dc.contributor.author	Fuller, MA
dc.contributor.author	Wood, K
dc.contributor.author	Holt, SA
dc.contributor.author	Köper, I
dc.date.accessioned	2022-09-11T21:42:20Z
dc.date.available	2022-09-11T21:42:20Z
dc.date.issued	2018-05-09
dc.identifier.citation	Phys Chem Chem Phys, 2018, 20, (18), pp. 12958-12969
dc.identifier.issn	1463-9076
dc.identifier.issn	1463-9084
dc.identifier.uri	http://hdl.handle.net/10453/161694
dc.description.abstract	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria. The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules. The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated. By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane. The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin. A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.
dc.format	Print
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation.ispartof	Phys Chem Chem Phys
dc.relation.isbasedon	10.1039/c8cp01346b
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 09 Engineering
dc.subject.classification	Chemical Physics
dc.subject.mesh	Biomimetic Materials
dc.subject.mesh	Cell Membrane
dc.subject.mesh	Colistin
dc.subject.mesh	Dielectric Spectroscopy
dc.subject.mesh	Electric Capacitance
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Lipopolysaccharides
dc.subject.mesh	Neutron Diffraction
dc.subject.mesh	Phosphatidylcholines
dc.subject.mesh	Scattering, Small Angle
dc.subject.mesh	Surface Properties
dc.subject.mesh	Cell Membrane
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Colistin
dc.subject.mesh	Lipopolysaccharides
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Phosphatidylcholines
dc.subject.mesh	Neutron Diffraction
dc.subject.mesh	Electric Capacitance
dc.subject.mesh	Surface Properties
dc.subject.mesh	Biomimetic Materials
dc.subject.mesh	Scattering, Small Angle
dc.subject.mesh	Dielectric Spectroscopy
dc.title	A tethered bilayer lipid membrane that mimics microbial membranes.
dc.type	Journal Article
utslib.citation.volume	20
utslib.location.activity	England
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-11T21:42:17Z
pubs.issue	18
pubs.publication-status	Published
pubs.volume	20
utslib.citation.issue	18

Abstract:

A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria. The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules. The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated. By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane. The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin. A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.

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http://hdl.handle.net/10453/161694